Chonnam Med J.  2011 Aug;47(2):85-89. 10.4068/cmj.2011.47.2.85.

Establishment of a Cell Line (CNUH-HNSCC-1) Derived from an Advanced Laryngeal Squamous Cell Carcinoma

• Lee DH
• Lee JK

• KMID: 2048790
• DOI: http://doi.org/10.4068/cmj.2011.47.2.85

Abstract

Cancer cell lines are the basic material for various lines of cancer research. Diverse cancer cell lines derived from tissues of various head and neck regions are needed for biological research on head and neck cancer. However, cell lines derived from cancer of the head and neck are not common. Recently, we established and characterized a novel human squamous carcinoma cell line, CNUH-HNSCC-1. From six cases of head and neck cancer, we established one specimen that was maintained for over 50 passages. We characterized the cell line as follows: growth patterns and curve, morphology by use of phase-contrast microscopy, and tumorigenicity by implanting the cell line into nude mice and making morphological comparisons. CNUH-HNSCC-1 cells grew well in vitro even after passage 50. However, the cells failed to form tumors in nude mice. CNUH-HNSCC-1 cells could be used as a control cell line for studying the biology of head and neck cancer.

Keyword

Squamous cell carcinoma; Cell line; Head and neck neoplasms

MeSH Terms

Animals
Biology
Carcinoma, Squamous Cell
Cell Line
Head
Head and Neck Neoplasms
Humans
Mice
Mice, Nude
Microscopy, Phase-Contrast
Neck

Figure

• 

  FIG. 1 Growth curve of the cultured CNUH-HNSCC-1 cells at passage 50. Each point represents the mean value of triplicate cultures.

• 

  FIG. 2 Primary culture cells initially appeared as small and round single cells (A). Two days later, the cells formed branches (B), and were 60% confluent about 4 days later (C). About 7 days later, the cells were 100% confluent (D) (Magnification for all panels, 100×).

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  FIG. 3 A small round nodule (arrow) about 5×3 mm developed in the back of the nude mice 1 week after the injection with CNUH-HNSCC-1 cells.

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