Korean J Nephrol.  2006 Nov;25(6):913-921.

The Antiproteinuric Effects of Green Tea Polyphenol on Cyclosporine A-Induced Acute Renal Injury in Mice

• Shin BC
• Kim HL
• Chung JH

Abstract

BACKGROUND
It has been reported that there is association between cyclosporine (CsA) nephrotoxicity and proteinuria. The aim of this study was to investigate the anti-proteinuric effects of green tea polyphenol (GTP) on CsA-induced acute renal injury in mice.
METHODS
The mice (n=20) were divided into 4 groups (n=5/group); group 1 (control group) mice were intraperitoneally (IP) injected 0.9% saline, group 2 (CsA group) mice were IP injected CsA 50 mg/kg, group 3 (iNOS inhibitor group) mice were given in addition N(G)-nitro-L-arginine-methyl ester (L-NAME) 12 mmol/L by subcutaneous injection. Group 4 (GTP group) mice were given CsA by IP injection and GTP 100 mg/kg by subcutaneous injection.
RESULTS
Urine protein significantly increased in group 2 (28.6+/-11.1 g/kg/day) compared to group 1 (9.1+/-5.5 g/kg/day, p<0.01) and significantly decreased in group 4 (11.2+/-8.8 g/kg/day, p<0.01) compared to group 2. Renal tissue malondialdehyde level of group 2 significantly increased compared to group 1 and significantly decreased in GTP group (p<0.01).
CONCLUSION
This study proves that proteinuria of the CsA induced nephrotoxicity is associated with lipid peroxidation and nitric oxide production. GTP treatment has meaningful antiproteinuric effects through antioxidative effect in the kidney from CsA-induced acute renal injury.

Keyword

Green tea polyphenol; Cyclosporine; Proteinuria; Antioxidant

MeSH Terms

Acute Kidney Injury*
Animals
Cyclosporine*
Guanosine Triphosphate
Injections, Subcutaneous
Kidney
Lipid Peroxidation
Malondialdehyde
Mice*
Nitric Oxide
Proteinuria
Tea*
Cyclosporine
Guanosine Triphosphate
Malondialdehyde
Nitric Oxide
Tea