Korean J Lab Med.
2003 Feb;23(1):18-24.
Characterization and Prevalence of Escherichia coli and Klebsiella pneumoniae Isolates Producing an Extended-Spectrum beta-Lactamase from Korean Hospitals
- Affiliations
-
- 1Department of Orthopedic Surgery, Kosin University College of Medicine, Busan, Korea. kscpjsh@ns.kosinmed.or.kr
- 2Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea.
- 3Department of Neurosurgery, Kosin University College of Medicine, Busan, Korea.
- 4Department of Genetic Engineering, Youngdong University, Chungbuk, Korea.
- 5Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.
Abstract
- BACKGROUND
The aim of this study was to survey the nationwide susceptibilities of E. coli and K. pneumoniae against third generation cephalosporins and aztreonam in order to determine the prevalence of extended-spectrum beta-lactamase (ESBL)-producers and to characterize genotypes of ESBLs. METHODS: A total of 6, 567 E. coli and 2, 652 K. pneumoniae non-duplicate strains were isolated from 13 hospitals in April to June 2002. Antimicrobial susceptibilities were tested by the disk diffusion method. Twenty isolates of E. coli and 20 K. pneumoniae were collected from each hospital. ESBL production was determined by a double-disk synergy test. The ceftazidime-resistance of the ESBL-producers was transferred to azide-resistant E. coli J53 by conjugation. MICs of beta-lactam antibiotics to transconjugants were determined by the agar dilution method. Searches for blaTEM , blaSHV , blaCTX-M and blaCMY genes in transconjugants were performed by PCR amplification. RESULTS: Eighty-nine percents of E. coli and 71% of K. pneumoniae isolates were susceptible to ceftazidime. Nine percents of E. coli (23/249) and 30% (78/260) of K. pneumoniae isolates showed positive results in the double-disk synergy test. Ceftazidime-resistance of 13 (57%) E. coli and 42 (53%) K. pneumoniae isolates were transferred to E. coli J53 by conjugation. Among 55 transconjugants, 46 strains were resistant to ceftazidime, while only 16 strains were resistant to cefotaxime. Twelve transconjugants were also resistant to cefoxitin and cefotetan. Banding patterns of PCR amplification showed that the blaTEM , blaSHV , blaCTX-M and blaCMY genes were harboured by 44, 39, 4 and 5 transconjugants, respectively. CONCLUSIONS: E. coli and K. pneumoniae isolates producing TEM-, SHV-type, or CTX-M-type ESBLs are wide spread in Korean hospitals. The spread of ESBL genes could compromise the future usefulness of 3rd generation cephalosporins and aztreonam for the treatment of E. coli and K. pneumoniae infections.
Keyword
MeSH Terms
-
Agar
Anti-Bacterial Agents
Aztreonam
beta-Lactamases*
Cefotaxime
Cefotetan
Cefoxitin
Ceftazidime
Cephalosporins
Diffusion
Escherichia coli*
Genotype
Klebsiella pneumoniae*
Pneumonia
Polymerase Chain Reaction
Prevalence*
Agar
Anti-Bacterial Agents
Aztreonam
Cefotaxime
Cefotetan
Cefoxitin
Ceftazidime
Cephalosporins
beta-Lactamases
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- In Vitro Susceptibility of piperacillin/tazobactam Against extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae
- Prevalence of TEM- and SHV-type Beta-lactamase gene in Escherichia coli and Klebsiella pneumoniae in Korea
- Evaluation of the Method to Screen Isolates of Extended-Spectrum -Lactamase-Producing Klebsiella pneumoniae and Escherichia coli Using Cefpodoxime Disk
- Antibiogram of Escherichia coli and Klebsiella spp. Detected by Vitek ESBL Test
- Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae
