Lab Med Online.  2012 Jan;2(1):55-58. 10.3343/lmo.2012.2.1.10.

Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae

• Jin WY
• Jang SJ
• Kim YS
• Park G
• Moon DS
• Park YJ

• KMID: 1435754
• DOI: http://doi.org/10.3343/lmo.2012.2.1.10

Abstract

The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.

Keyword

Extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae; MicroScan; Phoenix; Double-disk synergy test

MeSH Terms

beta-Lactamases
Delivery of Health Care
Escherichia
Escherichia coli
Klebsiella
Klebsiella pneumoniae
Pneumonia
Polymerase Chain Reaction
beta-Lactamases

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