J Korean Orthop Res Soc.
2004 Oct;7(2):108-114.
ERK1/2 Activation Modulates Matrix Metalloproteinase 2 Activity and Ezrin Expression in Osteosarcoma Cell Lines
- Affiliations
-
- 1Brain Korea 21 project for medical science, Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, Korea. ljwos@yumc.yonsei.ac.kr
Abstract
- PURPOSE
In this study, we aimed to investigate the influence of mitogen-activated protein kinase (MAPK) signal pathway and related factors of metastatic property on the osteosarcoma cell lines.
MATERIALS AND METHODS
Osteosarcoma cell lines, MG-63, HOS, U2OS, SAOS-2, and osteoblast-like cell line, MC3T3-E1 were used for evaluating the activation of MAPK signal pathway and the effect of ERK1/2 inhibiton by 15 micrometer U0126 for 6 days. Then we measured the activity of matrix metalloproteinases (MMP) by gelatin zymography analysis.
RESULTS
ERK was strongly activated in three osteosarcoma cell lines, MG-63, HOS, U2OS, and was weakly activated in SAOS-2 cell line. MMP2 was strongly activated in MG-63, HOS, U2OS cell lines. However, SAOS-2 cell line, in which ERK1/2 was weakly activated, showed weak activation of MMP2. When the ERK1/2 was inhibited by U0126 for 6 days, the ezrin expression was remarkably suppressed in all osteosarcoma cell lines. These data showed that the suppression of ezrin expression might be dependent on the ERK1/2 activation in osteosarcoma cell lines.
CONCLUSION
Our results suggested that the ERK1/2 activation might be related to the activity of MMP2 and the suppression of ezrin expression in osteosarcoma cell lines. We suggest that ERK1/2 activation might be closely related to the ezrin expression and the ezrin expression might play an important role in the activation of MMP2 which is known to involve in the activated MMP2 involves in early metastatic properties in osteosarcoma.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Ezrin is an Essential Marker for Metastasis of Gynecologic Cancer
- Matrix metalloproteinases in human gliomas: activation of matrix metalloproteinase-2 (MMP-2) may be correlated with membrane-type-1 matrix metalloproteinase (MT1-MMP) expression
- The Expression of Matrix Metalloproteinase-9 and Tumor Angiogenesis in Human Osteosarcoma
- Collagen-induced Activation of MMPs ( Membrane -type Matrix Metalloproteinase and Matrix Metalloproteinase-2) in ovarian cancer cell lines in Vitro
- Role of NFkappaB in toll-like receptor 9-mediated matrix metalloproteinase-9 expression
