J Korean Cancer Assoc.
2000 Oct;32(5):852-862.
Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines
- Affiliations
-
- 1Departments of Surgery, School of Medicine, Ajou University, Suwon, Korea. hansu@madang.ajou.ac.kr
- 2Departments of Biochemistry, School of Medicine, Ajou University, Suwon, Korea.
- 3Department of Diagnostic Pathology, School of Medicine, Sung Kyun Kwan University,Samsung Medical Center, Seoul, Korea.
- 4Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.
Abstract
-
PURPOSE: Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied.
MATERIALS AND METHODS
Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells. Tyrosine phosphorylation of c-Met, E-cadherin, alpha-, beta-, gamma-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.
RESULTS
Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.
CONCLUSION
These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Role of Hepactocyte Growth Factor, Met, and E-cadherin in the Progression of Gastric Carcinomas
- Function of hepatocyte growth factor in gastric cancer proliferation and invasion
- Effect of EGF and TGF- on Cellular Adhesion and the Expression of E-cadherin and EGF Receptor in Cervical Cancer Cell Lines
- Correlation between the overexpression of epidermal growth factor receptor and mesenchymal makers in endometrial carcinoma
- Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines
