J Korean Cancer Assoc.  2000 Oct;32(5):852-862.

Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines

Affiliations
  • 1Departments of Surgery, School of Medicine, Ajou University, Suwon, Korea. hansu@madang.ajou.ac.kr
  • 2Departments of Biochemistry, School of Medicine, Ajou University, Suwon, Korea.
  • 3Department of Diagnostic Pathology, School of Medicine, Sung Kyun Kwan University,Samsung Medical Center, Seoul, Korea.
  • 4Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.

Abstract

PURPOSE: Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied.
MATERIALS AND METHODS
Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells. Tyrosine phosphorylation of c-Met, E-cadherin, alpha-, beta-, gamma-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.
RESULTS
Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.
CONCLUSION
These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin.

Keyword

Stomach neoplasm; Hepatocyte growth factor; c-Met gene; E-cadherin; Invasion

MeSH Terms

Blotting, Western
Cadherins*
Cell Adhesion
Cell Fractionation
Cell Line*
Cell Proliferation
Cytoplasm
Fibroblasts
gamma Catenin
Hand
Hepatocyte Growth Factor*
Hepatocytes*
Immunoprecipitation
Membranes
Phosphorylation
Stomach Neoplasms
Tyrosine
Cadherins
Hepatocyte Growth Factor
Tyrosine
gamma Catenin
Full Text Links
  • JKCA
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr