J Korean Med Sci.  2009 Feb;24(1):97-103. 10.3346/jkms.2009.24.1.97.

EMP3 Overexpression in Primary Breast Carcinomas is not Associated with Epigenetic Aberrations

Affiliations
  • 1Department of Radiation Oncology, Shandong Tumor Hospital, Shandong University, Shandong, China.
  • 2Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong, China. dryujinming@hotmail.com
  • 3Department of Neurosurgery, Qilu Hospital, Shandong University, Shandong, China.
  • 4Breast Center, Shandong Cancer Hospital and Institute, Shandong, China.
  • 5Department of Immunology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Shandong, China.
  • 6Cancer Center, Qilu Hospital, Shandong University, Shandong, China.

Abstract

Epithelial membrane protein 3 (EMP3) is a trans-membrane signaling molecule with important roles in the regulation of apoptosis, differentiation and invasion of cancer cells, but the detailed is largely still unknown. We analyzed the mRNA levels and methylation statuses of EMP3 in 63 primary breast carcinomas and assessed their correlations with clinicopathologic variables. The expression of EMP3 mRNA in primary breast carcinomas was significantly higher than the expression of 20 normal breast tissues (p<10(-7)). EMP3 overexpression in breast carcinomas was significantly related to histological grade III (p=3.9X10(-7)), lymph node metastasis (p= 0.003), and strong Her-2 expression (p=3.3X10(-6)). Hypermethylation frequencies of EMP3 were detected in 36.5% of breast carcinomas by methylation-specific polymerase chain reaction. However, no significant correlations were found between methylation status of EMP3 and mRNA expression levels as well as other clinical parameters. In conclusion, EMP3 may be a novel marker of tumor aggressiveness. Overexpression of EMP3 in primary breast carcinoma is not associated with DNA methylation.

Keyword

PMP22/EMP/MP20 Gene Family; Metastasis; Breast Neoplasms; DNA Methylation; Epigenetics, Genetic

MeSH Terms

Adult
Breast Neoplasms/*genetics/pathology
Carcinoma/*genetics/pathology
DNA Methylation
Epigenesis, Genetic
Female
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
Membrane Glycoproteins/*genetics/metabolism
Middle Aged
Neoplasm Staging
RNA, Messenger/metabolism
Receptor, erbB-2/genetics/metabolism
Severity of Illness Index
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