Korean J Intern Med.  2010 Mar;25(1):93-100. 10.3904/kjim.2010.25.1.93.

Ethyl Acetate Fraction from Cudrania Tricuspidata Inhibits IL-1beta-Stimulated Osteoclast Differentiation through Downregulation of MAPKs, c-Fos and NFATc1

• Lee EG
• Yun HJ
• Lee SI
• Yoo WH

• KMID: 1785661
• DOI: http://doi.org/10.3904/kjim.2010.25.1.93

Abstract

BACKGROUND/AIMS
The present study was performed to determine the effects of the ethyl acetate extract of Cudrania tricuspidata (EACT) on interleukin (IL)-1beta-stimulated receptor activator of NF-kappaB ligand (RANKL)-mediated osteoclast differentiation.
METHODS
Bone marrow cells were harvested from 6-week-old male imprinting control region mice, and the differentiation of osteoclasts from these cells was evaluated by tartrate-resistant acid phosphatase and resorption pit formation assay. Phosphorylated extracellular signal regulated kinase (p-ERK), phosphorylated p38, phosphorylated c-Jun amino-terminal kinase, NF-kappaB (p65), IkappaBalpha, c-Fos, and nuclear factor of activated T-cells c1 (NFATc1) expression was examined by immunoblotting and quantitative reverse transcription-polymerase chain reaction.
RESULTS
EACT inhibits IL-1beta-stimulated RANKL-mediated osteoclast differentiation. EACT also inhibits IL-1beta-stimulated RANKL-mediated phosphorylation of ERK 1/2, p38 mitogen activated protein kinase, and expression of c-Fos and NFATc1.
CONCLUSIONS
These results suggest that EACT may be involved in the inhibition of bone loss by preventing osteoclast formation and may be used to manage bone destruction in inflammatory diseases, such as rheumatoid arthritis.

Keyword

Osteoclast; Cell differentiation; RANK ligand; Interleukin-1beta

MeSH Terms

*Acetates
Animals
Bone Marrow Cells/cytology/drug effects/metabolism
Cell Differentiation/drug effects/physiology
Cell Survival/drug effects/physiology
Cells, Cultured
Down-Regulation/drug effects
Extracellular Signal-Regulated MAP Kinases/metabolism
Interleukin-1beta/*pharmacology
MAP Kinase Signaling System/*drug effects/physiology
Male
Mice
Mice, Inbred ICR
*Moraceae
NFATC Transcription Factors/metabolism
*Osteoclasts/cytology/drug effects/metabolism
Plant Extracts/*pharmacology
Proto-Oncogene Proteins c-fos/metabolism
RANK Ligand/metabolism
Stem Cells/cytology/drug effects/metabolism
p38 Mitogen-Activated Protein Kinases/metabolism