J Vet Sci.  2014 Jun;15(2):179-185. 10.4142/jvs.2014.15.2.179.

Effects of resveratrol on the insulin signaling pathway of obese mice

Affiliations
  • 1Department of Toxicology & Biochemistry, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. chhan@jejunu.ac.kr
  • 2Department of Internal Medicine, School of Medicine, Institute of Medical Science, Jeju National University, Jeju 690-756, Korea.

Abstract

The present study was conducted to investigate the effects of resveratrol on the insulin signaling pathway in the liver of obese mice. To accomplish this, we administered resveratrol to high fat diet-induced obese mice and examined the levels of protein phosphorylation in the liver using an antibody array. The phosphorylation levels of 10 proteins were decreased in the high fat diet and resveratrol (HFR) fed group relative to the levels in the high fat diet (HF) fed group. In contrast, the phosphorylation levels of more than 20 proteins were increased in the HFR group when compared with the levels of proteins in the HF group. Specifically, the phosphorylation levels of Akt (The308, Tyr326, Ser473) were restored to normal by resveratrol when compared with the levels in the HF group. In addition, the phosphorylation levels of IRS-1 (Ser636/Ser639), PI-3K p85-subunit alpha/gamma(Tyr467/Tyr199), PDK1 (Ser241), GSK-3alpha (S21) and GSK-3 (Ser9), which are involved in the insulin signaling pathway, were decreased in the HF group, whereas the levels were restored to normal in the HFR group. Overall, the results show that resveratrol restores the phosphorylation levels of proteins involved in the insulin signaling pathway, which were decreased by a high fat diet.

Keyword

Akt phosphorylation; antibody array; insulin signaling pathway; obese mice; resveratrol

MeSH Terms

Animals
Anti-Inflammatory Agents/*pharmacology
Fluorescent Antibody Technique
Insulin/*physiology
Liver/*metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Phosphorylation
Proteins/metabolism
Signal Transduction/*drug effects
Stilbenes/*pharmacology
Anti-Inflammatory Agents
Insulin
Proteins
Stilbenes
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