Korean J Intern Med.  2012 Sep;27(3):327-337. 10.3904/kjim.2012.27.3.327.

Interleukin-6 -634 C/G and -174 G/C Polymorphisms in Korean Patients Undergoing Hemodialysis

• Ryu JH
• Kim SJ

• KMID: 1776525
• DOI: http://doi.org/10.3904/kjim.2012.27.3.327

Abstract

BACKGROUND/AIMS
Chronic inflammatory status is a possible risk factor for vascular access dysfunction in hemodialysis (HD) patients, but susceptibility differences appear among individuals. Interleukin (IL)-6 is a well-known inflammatory cytokine with various polymorphisms. We examined whether IL-6 polymorphisms are associated with vascular access dysfunction in HD patients.
METHODS
A total of 80 HD patients (including 42 diabetic patients) were enrolled. Polymorphisms in the IL-6 gene promoter (-634 C/G and -174 G/C) were studied using restriction length polymorphism polymerase chain reaction analysis. Vascular access patency was compared between the patient groups with respect to IL-6 polymorphisms. An additional 89 healthy individuals were enrolled in the control group. Plasma IL-6 levels were de termined by enzyme-linked immunosorbent assay.
RESULTS
The GG genotype and G allele at position -634 in the IL-6 promoter were more frequently observed in HD patients than in controls. Furthermore, the distribution of the -634 polymorphism differed according to vascular access patency in non-diabetic HD patients. However, the G allele was not a significant risk factor for early access failure. No significant association appeared between the IL-6 -634 C/G polymorphism and plasma IL-6 levels. The C allele of the IL-6 -174 G/C polymorphism was not detected in our study population.
CONCLUSIONS
The IL-6 -634 G allele appears with greater frequently in patients with end-stage renal disease and may be associated with vascular access dysfunction in non-diabetic HD patients.

Keyword

Hemodialysis; Inflammation; Interleukin-6 polymorphism; Vascular access failure

MeSH Terms

Adult
Aged
Arteriovenous Shunt, Surgical/*adverse effects
Asian Continental Ancestry Group/*genetics
Case-Control Studies
Chi-Square Distribution
Enzyme-Linked Immunosorbent Assay
Female
Gene Frequency
Genotype
Graft Occlusion, Vascular/blood/ethnology/*genetics/physiopathology
Humans
Interleukin-6/blood/*genetics
Kidney Failure, Chronic/blood/ethnology/genetics/immunology/*therapy
Logistic Models
Male
Middle Aged
Odds Ratio
Phenotype
Polymerase Chain Reaction
*Polymorphism, Genetic
Promoter Regions, Genetic
*Renal Dialysis
Republic of Korea
Time Factors
Treatment Outcome
Vascular Patency/*genetics