Korean J Physiol Pharmacol.  2012 Jun;16(3):219-224. 10.4196/kjpp.2012.16.3.219.

Gap Junction Contributions to the Goldfish Electroretinogram at the Photopic Illumination Level

Affiliations
  • 1Natural Sciences Section, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. cjung@catholic.ac.kr

Abstract

Understanding how the b-wave of the electroretinogram (ERG) is generated by full-field light stimulation is still a challenge in visual neuroscience. To understand more about the origin of the b-wave, we studied the contributions of gap junctions to the ERG b-wave. Many types of retinal neurons are connected to similar and different neighboring neurons through gap junctions. The photopic (cone-dominated) ERG, stimulated by a small light beam, was recorded from goldfish (Carassius auratus) using a corneal electrode. Data were obtained before and after intravitreal injection of agents into the eye under a photopic illumination level. Several agents were used to affect gap junctions, such as dopamine D1 and D2 receptor agonists and antagonists, a nitric oxide (NO) donor, a nitric oxide synthase (NOS) inhibitor, the gap junction blocker meclofenamic acid (MFA), and mixtures of these agents. The ERG b-waves, which were enhanced by MFA, sodium nitroprusside (SNP), SKF 38393, and sulpiride, remained following application of a further injection of a mixture with MFA. The ERG b-waves decreased following NG-nitro-L-arginine methyl ester (L-NAME), SCH 23390, and quinpirole administration but were enhanced by further injection of a mixture with MFA. These results indicate that gap junction activity influences b-waves of the ERG related to NO and dopamine actions.

Keyword

Electroretinogram; b-wave; Gap-junction; Nitric oxide; Dopamine

MeSH Terms

2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Benzazepines
Dopamine
Electrodes
Eye
Gap Junctions
Goldfish
Humans
Intravitreal Injections
Light
Lighting
Meclofenamic Acid
Neurons
Neurosciences
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Nitroprusside
Quinpirole
Retinal Neurons
Sulpiride
Tissue Donors
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Benzazepines
Dopamine
Meclofenamic Acid
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Nitroprusside
Quinpirole
Sulpiride

Figure

  • Fig. 1 A schematic drawing, as seen from the side, of the overall process of the light stimulus for electroretinogram (ERG) measurements and consequent responses. (A) The dashed and dotted lines show a sketch of the ray paths of the smaller and larger white circle beams emitted from the projector, respectively. a, computer; b, beam projector; c, surface mirror; d, eye ball; e, iris; f, retina (shaded area); g, recording electrode; h, differential amplifier. (B) The ERG responses were evoked by small and large sizes of circle light beam before (upper and down, left) and after (upper and down, right) intravitreal injection of 200 µM MFA. The small and large sizes of the light beam imaged on the surface of the mirror were 6 mm and 60 mm in diameter corresponding to the values of 0.012° and 0.12° in the field of view. Light stimulus was a 4-s presented by the square wave. The components of ERG a-, b-, c-, and d-waves are denoted as a, b, c, and d.

  • Fig. 2 Modulation of the b-wave. (A) The b-wave was recorded under the control condition, after injecting 1 mM SNP, and after injecting a mixture of 1 mM SNP and 200 µM MFA. (B) The b-wave was recorded under the control condition, after injecting 2 mM L-NAME, and after injecting a mixture of 2 mM L-NAME and 200 µM MFA. In bar graphs end of the each low line, the mean peak amplitudes of the b-wave by drugs are plotted as a percentage of the peak response under control condition. In this and all subsequent figures, each bar represents the mean±standard error and single (p<0.05) and double stars (p<0.01) above the data column indicate significant and very significant statistical differences, respectively. See text for details of the statistical comparisons.

  • Fig. 3 Modulation of b-waves. (A) b-waves were recorded under the control condition, after injecting 100 µM SKF 38393, and after injecting a mixture of 100 µM SKF 38393 and 200 µM MFA. (B) b-waves were recorded under the control condition, after injecting 30 µM SCH 23390, and after injecting a mixture of 30 µM SCH 23390 and 200 µM MFA.

  • Fig. 4 Modulation of b-waves. (A) b-waves were recorded under the control condition, after injecting 100 µM quinpirole, and after injecting a mixture of 100 µM quinpirole and 200 µM MFA. (B) b-waves were recorded under the control condition, after injecting 200 µM sulpiride, and after injecting a mixture of 200 µM sulpiride and 200 µM MFA. (C) b-waves were recorded under the control condition, after injecting 200 µM dopamine, and after injecting a mixture of 200 µM dopamine and 200 µM MFA.


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