Korean J Pediatr.  2005 Jun;48(6):649-654.

Near-Infrared Spectroscopy for Monitoring Cerebral Hemodynamics in Hyperbilirubinemia-induced Newborn Piglets

• Hwang JH
• Choi CW
• Chang YS
• Park WS

Abstract

PURPOSE
The present study examined how changes in cerebral hemodynamics in newborn piglets with bilirubin infusion can be evaluated by near infrared sepctroscopy (NIRS). METHODS: Seventeen newborn piglets were randomly divided into the following three experimental groups: six in the control group (CG) ; seven in the bilirubin infusion group (BG), and four in the bilirubin infusion with 7-nitroindazole group (NG). To achieve the concentration of bilirubin above 20 mg/dL, we injected a bolus of 40 mg/kg of bilirubin intravenously, followed by 30 mg/kg/hr of bilirubin continuous intravenous infusion. All groups were monitored with cerebral hemodynamics using near infrared spectroscopy (NIRS) and their brain cortexes were harvested and the activities of Na+, K+ -ATPase, level of conjugated dienes, ATP and phosphocreatine (PCr) were determined biochemically. RESULTS: No changes took place in CG. In BG and NG, base excess, pH, and MABP decreased, and lactate level in blood increased. Cerebral Na+, K+-ATPase activity and ATP, PCr level in BG significantly decreased and conjugated dienes increased compared to CG. These abnormalities observed in the BG were significantly improved in the NG. In continuous NIRS monitoring, [HbO2], [HbT], and [HbD] in BG were significantlly decreased compared to CG. However these abnormalities between NG and CG were not significantly different. There were no significant differences in ScO2 between the study groups. CONCLUSION: Our study suggests cerebral hemodynamic changes could be monitored by non- invasive NIRS in newborn piglets with bilirubin infusion.

Keyword

Jaundice; Hyperbilirubinemia; Kernicterus; Near infrared spectroscopy; Piglet

MeSH Terms

Adenosine Triphosphate
Bilirubin
Brain
Hemodynamics*
Humans
Hydrogen-Ion Concentration
Hyperbilirubinemia
Infant, Newborn*
Infusions, Intravenous
Jaundice
Kernicterus
Lactic Acid
Phosphocreatine
Polymerase Chain Reaction
Spectroscopy, Near-Infrared*
Spectrum Analysis
Adenosine Triphosphate
Bilirubin
Lactic Acid
Phosphocreatine