J Korean Acad Periodontol.  2009 Jun;39(2):119-128. 10.5051/jkape.2009.39.2.119.

Investigation of postnatal stem cells from canine dental tissue and bone marrow

Affiliations
  • 1Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Korea. lee@snu.ac.kr
  • 2Department of Dentistry, Hanil General Hospital, Korea.
  • 3Department of Periodontics, Asan Medical Center, Korea.

Abstract

PURPOSE: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow.
METHODS
Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation.
RESULTS
PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /beta tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation.
CONCLUSIONS
This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration.

Keyword

stem cells; differentiation; periodontal ligament; regeneration

MeSH Terms

Adipocytes
Animals
Antigens, Surface
Bone Marrow
Calcium Phosphates
Cell Differentiation
Chondrocytes
Durapatite
Mesenchymal Stromal Cells
Mice
Mice, Nude
Osteoblasts
Osteogenesis
Periodontal Ligament
Regeneration
Stem Cells
Transplants
Antigens, Surface
Calcium Phosphates
Durapatite
Full Text Links
  • JKAPE
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr