Lab Anim Res.  2013 Dec;29(4):212-220. 10.5625/lar.2013.29.4.212.

Precautionary effects of Red Liriope platyphylla on NGF secretion and Abeta42 deposition under the preclinical stage of Alzheimer's disease in Tg2576 mice

• Choi SI
• Go J
• Kim JE
• Lee YJ
• Kwak MH
• Jung YJ
• Hwang DY

• KMID: 1716216
• DOI: http://doi.org/10.5625/lar.2013.29.4.212

Abstract

Red Liriope platyphylla (RLP) has been manufactured from Liriope platyphylla (L. platyphylla, LP) roots using steaming process and investigated as a curative agent for treatment of diabetes, obesity and neurodegenerative disorders. To examine the precautionary effects of aqueous extract RLP (AEtRLP) on the preclinical stages of Alzheimer's Disease (AD), alterations of the key factors influencing AD were investigated in Tg2576 mice after AEtRLP7 treatment for 4 months. Abeta-42 peptides level was significantly decreased in the brain of AEtRLP7-treated Tg2576 mice compared to vehicle-treated Tg2576 mice, although significant differences on improving behavioral defects were not observed in the same group. The concentration of nerve growth factor (NGF) in serum was also higher in AEtRLP7-treated Tg2576 mice than vehicle-treated Tg2576 mice. However, the phosphorylation of TrkA and Erk among the downstream effectors of the high affinity NGF receptor was significantly lower in AEtRLP7-treated Tg2576 mice. A similar pattern was observed in the expression level of downstream effectors within low affinity NGF receptor. Overall, these results suggest that AEtRLP7 can contribute to preventing the production and deposition of Abeta-42 peptides during the early progression stage of AD in the brain of Tg2576 mice through increased NGF secretion.

Keyword

Red Liriope platyphylla; NGF; NGF signaling pathway; Abeta-42

MeSH Terms

Alzheimer Disease*
Animals
Brain
Mice*
Nerve Growth Factor*
Neurodegenerative Diseases
Obesity
Peptides
Phosphorylation
Receptor, Nerve Growth Factor
Steam
Nerve Growth Factor
Peptides
Receptor, Nerve Growth Factor
Steam

Figure

• 

  Figure 1 Effects of AEtRLP7 treatment on behavioral defects and Aβ-42 peptides production in Tg2576 mice. (A) To identify the inserted APPsw gene in Tg2576 mice, genomic DNA isolated from the tails of founder mice was analyzed by PCR. The resulting products (466-bp) are shown. (B) Alterations of behavioral defects in Tg2576 mice were detected by passive avoidance test at 3, 5 and 7 months. (C) Production of Aβ-42 peptide in Non-Tg, vehicle- and AEtRLP7-treated Tg2576 mice was observed in brain sections stained with anti-Aβ-42 antibody. (D) The concentration of Aβ-42 peptide in the brain tissue of Tg2576 mice was measured using an anti-Aβ-42 ELISA kit. Data are reported as the mean±SD of three experiments. a, P<0.05 relative to Non-Tg mice. b, P<0.05 relative to vehicle-treated Tg2576 mice.

• 

  Figure 2 Alteration on NGF secretion of Tg2576 mice. The concentration of NGF in the serum of Tg2576 mice was measured using an anti-NGF ELISA kit. This kit has 10-15 pg/mL of sensitivity and can detect NGF at 10 pg/mL to 1,000 pg/mL. Data are reported as the mean±SD of three experiments. a, P<0.05 relative to Non-Tg mice. b, P<0.05 relative to vehicle-treated Tg2576 mice.

• 

  Figure 3 Effects of AEtRLP7 treatment on the NGF receptor TrkA signaling pathway of Tg2576 mice. Total tissue lysates were prepared from the brain of Tg2576 mice treated with vehicle or AEtRLP7 as described in the Materials and Methods section. Fifty micrograms of protein per sample were immunoblotted with antibodies for each protein. Three samples were assayed in triplicate by Western blotting. Data are reported as the mean±SD. a, P<0.05 relative to Non-Tg mice. b, P<0.05 relative to vehicle-treated Tg2576 mice.

• 

  Figure 4 Effects of AEtRLP7 treatment on the NGF receptor p75NTR signaling pathway of Tg2576 mice. Total tissue lysates were prepared from the brain of Tg2576 mice treated with vehicle or AEtRLP7 as described in the Materials and Methods. Fifty micrograms of protein per sample was immunoblotted with antibodies for each protein. Three samples were assayed in triplicate by Western blot analysis. Data are reported as the mean±SD. a, P<0.05 relative to Non-Tg mice. b, P<0.05 relative to vehicle-treated Tg2576 mice.

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