J Korean Med Sci.  2007 Oct;22(5):815-819. 10.3346/jkms.2007.22.5.815.

House Dust Mite Induces Expression of Intercellular Adhesion Molecule-1 in EoL-1 Human Eosinophilic Leukemic Cells

• Kwon BC
• Sohn MH
• Kim KW
• Kim ES
• Kim KE
• Shin MH

• KMID: 1713286
• DOI: http://doi.org/10.3346/jkms.2007.22.5.815

Abstract

The house dust mite (HDM) is considered to be the most common indoor allergen associated with bronchial asthma. In this study, we investigated whether crude extract of the HDM Dermatophagoides farinae could activate human eosinophilic leukemic cells (EoL-1) to induce upregulation of cell-surface adhesion molecules. When EoL-1 cells were incubated with D. farinae extract, expression of intercellular adhesion molecule-1 (ICAM-1) significantly increased on the cell surfaces compared to cells incubated with medium alone. In contrast, surface expression of CD11b and CD49d in EoL-1 cells was not affected by D. farinae extract. In addition, pretreatment of cells with NF- kappaB inhibitor (MG-132) or JNK inhibitor (SP600125) significantly inhibited ICAM-1 expression promoted by HDM extract. However, neither p38 MAP kinase inhibitor nor MEK inhibitor prevented HDM-induced ICAM-1 expression in EoL-1 cells. These results suggest that crude extract of D. farinae induces ICAM-1 expression in EoL-1 cells through signaling pathways involving both NF- kappaB and JNK.

Keyword

Pyroglyphidae; EoL-1 Cells; Intercellular Adhesion Molecule-1; NF-KappaB

MeSH Terms

Animals
Anthracenes/pharmacology
Antigens, CD11b/biosynthesis
Cell Line, Tumor
Cell Membrane/metabolism
Eosinophils/*metabolism
Flow Cytometry/methods
*Gene Expression Regulation
Humans
Integrin alpha4/biosynthesis
Intercellular Adhesion Molecule-1/*metabolism
Leukemia/*metabolism
Leupeptins/pharmacology
Mitogen-Activated Protein Kinase 8/metabolism
NF-kappa B/metabolism
Pyroglyphidae
p38 Mitogen-Activated Protein Kinases/metabolism

Figure

• 

  Fig. 1 Effect of TNF-α and HDM extract on the expression of cell surface ICAM-1 on EoL-1 cells. EoL-1 cells were incubated with or without HDM extract (5-200 µg/mL) for 4.5 hr. TNF-α (20 ng/mL) was used as a positive control. Data are expressed as the mean±SEM of triplicate experiments. *, p<0.05 compared to cells incubated with medium alone.

• 

  Fig. 2 Effect of MG-132 on HDM extract-induced ICAM-1 expression on EoL-1 cells. Results are expressed as the mean±SEM from four different experiments. *, p<0.05 compared to cells pretreated cells with DMSO.

• 

  Fig. 3 Effect of various inhibitors of MAPKs on HDM extract-induced ICAM-1 expression on EoL-1 cells. Results are expressed as the mean±SEM from four different experiments. *, p<0.05 compared to cells pretreated with DMSO.

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