Korean J Pediatr.
2005 Aug;48(8):881-885.
Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells
- Affiliations
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- 1Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea. kekim@yumc.yonsei.ac.kr
- 2Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
- 3Department Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
- 4Department Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, Korea.
- 5BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
Abstract
- PURPOSE
Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils. METHODS: Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1. RESULTS: Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium. CONCLUSION: We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
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