Korean J Ophthalmol.  2013 Oct;27(5):368-371. 10.3341/kjo.2013.27.5.368.

Human Papilloma Virus in Retinoblastoma Tissues from Korean Patients

• Ryoo NK
• Kim JE
• Choung HK
• Kim N
• Lee MJ
• Khwarg SI

• KMID: 1707291
• DOI: http://doi.org/10.3341/kjo.2013.27.5.368

Abstract

PURPOSE
Recent reports suggest the association of human papilloma virus (HPV) with retinoblastoma. This study was performed to elucidate whether HPV infection is related to retinoblastoma among Koreans.
METHODS
A total of 54 cases diagnosed with retinoblastoma were enrolled from Seoul National University Children's Hospital and Seoul Metropolitan Government-Seoul National University Boramae Medical Center. Presence of human papilloma viral DNA was detected by in situ hybridization in formalin-fixed paraffin-embedded retinoblastoma tissues using both probes against high- and low risk HPV types.
RESULTS
The mean age at diagnosis was 22.0 months (range, 1.1 to 98.0 months), and the mean age at enucleation was 27.8 months (range, 1.5 to 112.7 months) among the 54 patients with retinoblastoma. HPV was not detected in any of the retinoblastoma samples using either high risk or low risk HPV probes.
CONCLUSIONS
Our study, being the first study in the Korean population, proposes that HPV infection may have no causal relationship with retinoblastoma in Koreans.

Keyword

DNA; Human papillomavirus; In situ hybridization; Retinoblastoma

MeSH Terms

Child, Preschool
DNA, Viral/*analysis
Eye Infections, Viral/complications/diagnosis/*epidemiology
Female
Humans
In Situ Hybridization
Incidence
Infant
Male
Papillomaviridae/*genetics
Papillomavirus Infections/complications/diagnosis/*epidemiology
Prevalence
Prognosis
Republic of Korea/epidemiology
Retinal Neoplasms/complications/pathology/*virology
Retinoblastoma/pathology/*virology
DNA, Viral

Figure

• 

  Fig. 1 (A,B) Hematoxylin and eosin staining confirm the presence of retinoblastoma cells, ×200, ×400, respectively. (C) In situ hybridization for human papilloma virus (HPV) in retinoblastoma tumor cells is negative for HPV-DNA. (D) Squamous cell carcinoma of the uterine cervix stain positive for HPV-DNA. Dark purple staining (depicted in black arrows) indicates integrated or episomal HPV-DNA.

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