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Effect of Fixation Time and Freeze-thaw Cycles on the Molecular Analysis of Viral DNA

Kim OJ

  • KMID: 1089415
  • J Vet Sci.
  • 2003 Aug;4(2):203-204.
This study was done to determine the effect of fixation time and freeze-thaw (FT) cycles on the quantitation and positivity of viral DNA. There were significant decreases in the viral...
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Factors Associated with HIV-1 Proviral DNA Loads in Patients with Undetectable Plasma RNA Load

Choi JY, Song YG, Kim YH, Kim CO, Kim MS, Chin BS, Han SH, Choi SH, Lee HS, Jeong SJ, Choi H, Kim JM

To evaluate factors associated with human immunodeficiency virus type 1 (HIV-1) proviral DNA load, we conducted a cross-sectional study of 36 chronically HIV-1- infected individuals with undetectable plasma viral RNA....
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Clinical significance of human papillomavirus genotyping

Choi YJ, Park JS

Cervical cancer is the fourth most common cancer in women worldwide, and the human papillomavirus (HPV) is the main causative agent for its development. HPV is a heterogeneous virus, and...
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Relevancy between Liver Injury, Serum HBV-DNA, and Intrahepatic HBcAg in Young Male Chronic HBV Carriers

Kim TH, Kim YS, Yeom JJ, Cho EY, Kim HS, Kim HC, Park DS, Cho JH, Yoon GJ, Moon HB

  • KMID: 1113322
  • Korean J Gastroenterol.
  • 2004 Aug;44(2):84-92.
BACKGROUND/AIMS: Although the viral load is correlated with HBcAg, liver injury was not correlated to viral load in HBeAg positive patient. We aimed to study the inter-relationship of clinical...
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Absence of Human Papillomavirus DNA in Nongenital Seborrheic Keratosis

Lee ES, Whang MR, Kang WH

Seborrheic keratosis (SK) is a benign epidermal tumor of unknown etiology. Because of its wart-like morphology, human papillomavirus (HPV) has been suggested as a possible causative agent. Viral involvement, however,...
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HBV DNA Levels, Aminotransferase and Histological Activity in Young Male Patients with HBeAg Positive Chronic Hepatitis B

Cho SC, Lee SH, Shinn JJ, Han SH, Roh BJ, Sohn JH, Lee DH, Kee CS

  • KMID: 863067
  • Korean J Hepatol.
  • 2002 Mar;8(1):44-51.
BACKGROUND/AIM: A significant correlation between HBV DNA and liver damage was found in precore mutant strains but there was no significant association between viral replication and liver damage in HBeAg...
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Human Papilloma Virus in Retinoblastoma Tissues from Korean Patients

Ryoo NK, Kim JE, Choung HK, Kim N, Lee MJ, Khwarg SI

PURPOSE: Recent reports suggest the association of human papilloma virus (HPV) with retinoblastoma. This study was performed to elucidate whether HPV infection is related to retinoblastoma among Koreans. METHODS: A total...
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The Clinical Significance of Quantitation of HBV DNA in Serum: Comparison of the Branched-DNA Assay with the Second-Generation Digene Hybrid Capture Assay and Long-term Observation

Jun EJ, Han JY, Choi H, Chang UI, Lee TK, Kim YH, Kim JI, Park SH, Kim JK, Chung KW, Sun HS

  • KMID: 1123165
  • Korean J Hepatol.
  • 2002 Jun;8(2):157-166.
BACKGROUNDS/AIMS: Serum levels of hepatitis B virus (HBV) DNA are direct measures of viral replication in hepatocytes. We compared branched-DNA assay (QuantiplexTM, bDNA) and Hybridization Capture II (HCII), and evaluated...
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Change of Hepatitis B Virus DNA Status in Anti-HBc Positive Liver Graft

Kwon CH, Suh KS, Cho JY, Yi NJ, Jang JJ, Lee KU

  • KMID: 760646
  • Korean J Hepatol.
  • 2006 Jun;12(2):191-200.
BACKGROUND/AIMS: Many patients with positive anti-HBc, but negative HBsAg, are known to harbor occult HBV infection, which may transmit the virus through the graft in liver transplantation. We examined...
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Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes

Park KS, Kim JY, Ki CS, Lee NY

BACKGROUND: We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide...
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Comparative Analysis of Polymerase Chain Reaction Assay for Herpes Simplex Virus 1 Detection in Tear

Lee SY, Kim MJ, Kim MK, Wee WR

PURPOSE: To comparatively analyze the methodological efficacy of the polymerase chain reaction (PCR) assay for herpes simplex virus 1 (HSV) detection in tears. METHODS: This retrospective study reviewed the medical records...
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Detection of Intrahepatic HBV DNA in HBsAg-negative Liver Diseases

Kim YS, Jang JY, Eun SH, Cheon YK, Kim YS, Moon JH, Cho YD, Jin SY, Shim CS, Kim BS

  • KMID: 760647
  • Korean J Hepatol.
  • 2006 Jun;12(2):201-208.
BACKGROUNDS/AIMS: Occult HBV infection is characterized by the presence of HBV infection with undetectable HBsAg. This study was carried out to find out the frequency of HBV infection in...
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Comparison of VERSANT Hepatitis B Virus DNA 3.0 Assay with Digene Hybrid Capture II Hepatitis B Virus DNA Test in Relation to Clinical Status of Hepatitis B Virus

Song EY, Hur M, Lee KM, Yun Y, Lee CH, Choi YS, Lee KA, Roh EY

BACKGROUND: Some differences exist among various Hepatitis B virus (HBV) DNA quantification assays due to lack of standardization and besides clinical usefulness has not been firmly elucidated in Korean...
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Clinical Validation of AdvanSure GenoBlot Assay as Primary Screening and Test of Cure for Human Papillomavirus Infection

Kahng J, Oh EJ, Lee HN, Lee DW, Kim Y

BACKGROUND: Clinical specificity and sensitivity are essential factors in the adoption of a human papillomavirus (HPV) test as a primary screening tool and test of cure after treatment of cervical...
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Relationship of Oxidative Stress in Hepatitis B Infection Activity with HBV DNA and Fibrosis

Duygu F, Karsen H, Aksoy N, Taskin A

BACKGROUND: The aim of this study was to evaluate oxidative stress in various clinical forms of hepatitis B infection and to investigate its role in the development of the chronic...
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In situ hybridization study on human papillomavirus DNA expression in benign and malignant squamous lesions of the esophagus

Woo YJ, Yoon HK

Histologic changes suggesting HPV infection are occasionally found adjacent to squamous cell carcinoma or in squamous papilloma of the esophagus, but the relationship between HPV infection and benign and malignant...
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Prospective comparison of hybrid capture 2 and SPF₁₀-LiPA for carcinogenic human papillomavirus detection and risk prediction of cervical cancer: a population-based cohort study in China

Dong L, Feng RM, Zhang L, Xu XQ, Zhao XL, Wang MZ, Qiao YL, Zhao FH

OBJECTIVE: To investigate the extent of the cross-reactivity of hybrid capture 2 (HC2) assay and evaluate the potential effect of cross-reactivity on the long-term risk for cervical cancer and precancers. METHODS:...
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High-risk human papillomavirus genotype distribution and attribution to cervical cancer and precancerous lesions in a rural Chinese population

Zhao XL, Hu SY, Zhang Q, Dong L, Feng RM, Han R, Zhao FH

OBJECTIVE: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening...
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Evaluation of a Multiplex Real-time PCR Assay for the Detection of Respiratory Viruses in Clinical Specimens

Rheem I, Park J, Kim TH, Kim JW

  • KMID: 2434480
  • Ann Lab Med.
  • 2012 Nov;32(6):399-406.
BACKGROUND: In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using...
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Performance Evaluation of Real-Q Enterovirus Quantification Kit for Enterovirus by Real-time PCR

Song D, Kim SY, Jo SA, Hahm HI, Hwang SH, Lim YT, Kim HH, Chang CL, Lee EY

BACKGROUND: Molecular methods have enabled rapid diagnosis of aseptic meningitis and have reduced both unnecessary therapeutic interventions and medical costs. In this study, we evaluated the analytical performance of the...
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