Ann Lab Med.  2014 Jul;34(4):279-285. 10.3343/alm.2014.34.4.279.

Comparison of the Digene HPV Genotyping LQ Test and the PANArray HPV Genotyping Chip for Detection of High-Risk or Probable High-Risk Human Papillomavirus Genotypes

• Park KS
• Kim JY
• Ki CS
• Lee NY

• KMID: 1791935
• DOI: http://doi.org/10.3343/alm.2014.34.4.279

Abstract

BACKGROUND
We evaluated the performance of two different array-based techniques, a bead-based multiplex genotyping method (LQ; digene HPV Genotyping LQ Test, QIAGEN, Germany) and a DNA chip-based method using peptide nucleic acid probes (PANArray; PANArray HPV Genotyping Chip, Panagene, Korea), for detection of human papillomavirus (HPV) and genotyping of high-risk (HR) or probable high-risk (PHR) HPVs in healthy patients who visited a health-promotion center.
METHODS
We obtained 508 unselected, consecutive cervicovaginal swab specimens. All specimens were examined by using the PANArray and LQ tests. All HPV-positive samples were then analyzed by multiplex PCR and direct sequencing.
RESULTS
The LQ test detected 47 HPV-positive cases (9.3%) with HR or PHR genotypes and the PANArray test identified 36 cases (7.1%). When the results of LQ and PANArray were compared by using comprehensive genotyping (integrated interpretation of the results of LQ, PANArray, multiplex PCR, and direct sequencing) for the detection of HR or PHR genotypes, the kappa values were 0.44 and 0.30 for LQ and PANArray, respectively. In comparison to comprehensive genotyping, the LQ test yielded 53 (60.0%) concordant and 12 (13.5%) compatible results, and the PANArray yielded 36 (40.4%) concordant and three (3.4%) compatible results.
CONCLUSIONS
The results of the LQ test had higher concordance and/or greater compatibility with those of comprehensive genotyping for the detection of HR or PHR genotypes than those of the PANArray test.

Keyword

Human papillomavirus; DNA test; Comparison; Genotyping

MeSH Terms

Adult
Aged
Aged, 80 and over
DNA, Viral/*analysis
Genotype
Humans
Middle Aged
*Multiplex Polymerase Chain Reaction
*Oligonucleotide Array Sequence Analysis
Papillomaviridae/*genetics
Prospective Studies
Reagent Kits, Diagnostic
Sequence Analysis, DNA
DNA, Viral
Reagent Kits, Diagnostic

Figure

• 

  Fig. 1 Study design and results for the comparison of LQ and PANArray assays using comprehensive genotyping as a reference. Dotted line arrows represent comparative evaluation (LQ vs. comprehensive genotyping, PANArray vs. comprehensive genotyping).Abbreviations: HR, high-risk; PHR, probable high-risk; LR, low-risk; NEG, negative.

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