Ann Lab Med.  2012 Mar;32(2):113-118. 10.3343/alm.2012.32.2.113.

Relationship of Oxidative Stress in Hepatitis B Infection Activity with HBV DNA and Fibrosis

• Duygu F
• Karsen H
• Aksoy N
• Taskin A

• KMID: 1245250
• DOI: http://doi.org/10.3343/alm.2012.32.2.113

Abstract

BACKGROUND
The aim of this study was to evaluate oxidative stress in various clinical forms of hepatitis B infection and to investigate its role in the development of the chronic form of the disease.
METHODS
Ninety-three patients with inactive hepatitis B surface antigen (HbsAg) carrier state (IHBCS), 65 patients with chronic hepatitis B infection (CHB), and 42 healthy adults were included in the study. The following values were measured and compared in patient groups: total antioxidant status (TAS), total oxidative stress (TOS), oxidative stress index (OSI), sulfhydryl (SH), lipid peroxidation (LOOH), catalase (CAT), and ceruloplasmin. In patients with chronic hepatitis B, these values were compared with HBV DNA and fibrosis levels.
RESULTS
ALT, TOS, LOOH, and OSI levels were higher in the CHB group compared to the other groups (P<0.001). Catalase levels increased in the CHB and IHBCS groups compared to the control group (P<0.001). Total aminooxidant and ceruloplasmin levels were found to be lowest in the CHB group and highest in the control group (P<0.001). Sulfhyrdyl was higher in the control group compared to the other groups (P<0.001). In the CHB group, there was no correlation between the HBV DNA and OSI (P>0.05).
CONCLUSIONS
These finding suggested that oxidative stress is associated with hepatitis B activity.

Keyword

Hepatitis B; HBV DNA; Fibrosis; Oxidative stress; Catalase; Sulfhydryl

MeSH Terms

Adolescent
Adult
Aged
Alanine Transaminase/blood
Antioxidants/metabolism
Carrier State
Catalase/blood
DNA, Viral/*analysis
Female
Fibrosis
Hepatitis B/*metabolism/pathology
Hepatitis B Surface Antigens/blood
Hepatitis B virus/*genetics
Hepatitis B, Chronic/metabolism/pathology
Humans
Lipid Peroxidation
Male
Middle Aged
*Oxidative Stress
Sulfhydryl Compounds/blood
Young Adult

Figure

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  Fig. 1 Oxidative stress index values of patient groups and control group.

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  Fig. 2 Totally antioxidant status values of patient groups and control group.Abbreviation: TAX, totally antioxidant status.

• 

  Fig. 3 Ceruloplasmin values of patient groups and control group.

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