Korean J Nephrol.  2002 Mar;21(2):199-204.

Effects of Immune Aggregates, Hydrocortisone and Angiotensin II Receptor Blocker on Interleukin-6 mRNA Expression in Human Mesangial Cells

• Ihm CG
• Park JK
• Lee TW
• Cha DR

Abstract

PURPOSE: Various factors regulate interleukin(IL)-6 expression in mesangial cells (MCs). Immune complexes or angiotensin II(AII) are involved in the development of glomerulonephritis(GN). We evaluated the effects of IgG and IgA aggregates or AII on IL-6 mRNA expression in human MCs and the modulation by losartan, an AT1 receptor blocker, or hydrocortisone.
METHODS
After 48 hours of culture in the presence of sera, IL-6 mRNA expression was analyzed by reverse transcription and polymerase chain reaction(PCR).
RESULTS
Incubation of MCs with IgA or IgG aggregates(100 microgram/mL) as well as AII(10(-7) M) enhanced the ratio of PCR products for IL-6 to beta-actin on densitometric results. The addition of hydrocortisone(0.5 microgrammL) reduced the IgA aggregates-induced IL-6 mRNA expression and losartan(10(-6) M) reduced IgG aggregates- induced IL-6 mRNA expression.
CONCLUSION
These results suggest that IgG and IgA aggregates and AII may induce IL-6 expression in GN which can be partially suppressed by hydrocortisone or AT1 receptor blocker.

Keyword

Immune aggregates; Angiotensin II; Interleukin-6; AT1 blocker; Hydrocortisone

MeSH Terms

Actins
Angiotensin II*
Angiotensins*
Antigen-Antibody Complex
Humans*
Hydrocortisone*
Immunoglobulin A
Immunoglobulin G
Interleukin-6*
Losartan
Mesangial Cells*
Polymerase Chain Reaction
Receptors, Angiotensin*
Reverse Transcription
RNA, Messenger*
Actins
Angiotensin II
Angiotensins
Antigen-Antibody Complex
Hydrocortisone
Immunoglobulin A
Immunoglobulin G
Interleukin-6
Losartan
RNA, Messenger
Receptors, Angiotensin