Korean J Gastroenterol.  2001 Feb;37(2):90-97.

Effects of Daily Usual Dose of Cyclooxygenase-2 Selective and Non-selective Nonsteroidal Anti-inflammatory Drug on Gastric Mucosal Damage, Prostaglandin E2, Tumor Necrosis Factor-alpha and Neutrophil Infiltration

• Bhang CS
• Kim SS
• Song HJ
• Sung YJ
• Kim JI
• Han SW
• Chung IS
• Sun HS
• Park DH
• Yu JY

Abstract

BACKGROUND/AIMS: Recent studies suggested that neutrophil and tumor necrosis factor-alpha (TNF-alpha could play a significant role in NSAID-induced gastric mucosal damage. However, there is no data about the relationship between daily usual doses of NSAID and prostalglandin, neutrophil, or TNF-alpha
METHODS
We divided mice into 3 groups and administered indomethacin (3 mg/kg, indomethacin group), NS-398 (3 mg/kg, NS-398 group) or vehicle alone (control group) orally to each mice group for 7 days. Then, we evaluated the concentrations of prostaglandin E2 (PGE2) and TNF-alpha neutrophil infiltration and mucosal damage score for their gastric tissue.
RESULTS
Indomethacin group showed the lowest concentration of PGE2 and the highest values in TNF-alpha concentration, neutrophil infiltration and mucosal damage score (p<0.01). NS-398 group showed a lower PGE2 concentration and a higher microscopic mucosal damage score than the control group (p<0.01), but there were no differences in TNF-alpha concentration, neutrophil infiltration and gross mucosal damage scores. The PGE2 concentration was inversely related to TNF-alpha concentration (r=-0.768).
CONCLUSIONS
We suggest that decrease of prostaglandin concentration and increase of TNF-alpha production and neutrophil infiltration play important roles in NSAID-induced gastric mucosal damage. Cyclooxygenase-2 (COX-2) selective inhibitors, which have less effect on prostaglandin and TNF-alpha concentration and neutrophil infiltration, can reduce the gastric mucosal damage, as compared with the conventional NSAID.

Keyword

Prostaglandin; TNF-alpha ; Neutrophi; Cyclooxygenase-2

MeSH Terms

Animals
Cyclooxygenase 2*
Dinoprostone*
Indomethacin
Mice
Neutrophil Infiltration*
Neutrophils*
Tumor Necrosis Factor-alpha*
Cyclooxygenase 2
Dinoprostone
Indomethacin
Tumor Necrosis Factor-alpha