J Korean Orthop Res Soc.  2006 Apr;9(1):40-47.

Role of Tumor Necrosis Factor Alpha, Interleukin 8 and Dexamethasone in the FAK Expression by Human Nucleus Pulposus Cells

Affiliations
  • 1Department of Orthopedic Surgery, Cheju National University Hospital, Jeju, Korea.
  • 2Department of Orthopedic Surgery, Chonbuk National University Hospital, Jeonju, Korea. jhlee55@chonbuk.ac.kr

Abstract

PURPOSE
Human nucleus pulposus cells from intervertebral disc specimens were cultured to study the effects of TNF-alphaand IL-8 on the FAK expression by these cells. The effect of co-stimulation with dexamethasone on the FAK expression by nucleus pulposus cells was also studied.
MATERIALS AND METHODS
The FAK expression in cultured human nucleus pulposus cells relation to TNF-alpha, IL-8 and dexamethasone was assessed by performing western blot.
RESULTS
The activated FAK expression was up-regulated by treatments of TNF-alpha and IL-8. Dexamethasone attenuated the cytokine-induced FAK expression. The effects of inflammatory cytokines on the FAK expression were found to be concentration dependent with greater correlation shown by IL-8 than by TNF-alpha.
CONCLUSION
The FAK expression of human nucleus pulposus cells was up-regulated by TNF-alpha and IL-8 stimulation and it was attenuated by the co-administration of dexamethasone.

Keyword

Human nucleus pulposus; Focal adhesion kinase; Tumor necrosis factor alpha; Interleukin-8; Dexamethasone

MeSH Terms

Blotting, Western
Cytokines
Dexamethasone*
Focal Adhesion Protein-Tyrosine Kinases
Humans*
Interleukin-8
Intervertebral Disc
Tumor Necrosis Factor-alpha*
Cytokines
Dexamethasone
Focal Adhesion Protein-Tyrosine Kinases
Interleukin-8
Tumor Necrosis Factor-alpha
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