J Bacteriol Virol.  2005 Sep;35(3):183-190.

Detection of Antibiotic Resistant Genes in Salmonella enterica Serovar Typhimurium Isolated from Foodborne Patients in Seoul Using Multiplex-PCR

• Oh YH
• Song MO
• Kim MS
• Park SG
• Lee YK

Abstract

The frequency of antibiotic resistance among Salmonella enterica serovar Typhimurium has increased due to the transfer of multiple resistance factors. We detected the 13 antibiotic resistance genes by multiplex-PCR and compared with the results of phage typing and antibiotic disk diffusion for 49 S. typhimurium isolated from food-poisoning outbreaks in Seoul from 1999 to 2002. Resistance genes for tetracycline, streptomycin, ampicillin, sulfonamide, amino-glycoside-modifying enzyme, chloramphenicol, kanamycin, and trimethoprim were detected in 67.3%, 57.1%, 26.5%, 8.1%, 8.1%, 5%, 2.0%, and 0% of isolates, respectively. Overall 28 isolates (57.1%) possessed two or more antibiotic resistance genes. Class 1 integron carrying multidrug resistace genes, ant(3")-IaB, blaPSE, qacE delta1/sul, and tet G were amplified especially in only DT104 isolates. Among the related resistance genes for same antibiotics, strA and strB for streptomycin resistance were simultaneously detected but tetA and tetB for tetracycline were sporadically detected. DT 104 isolates contained only aadA2 and tetG.

Keyword

S. typhimurium; Antibiotic resistance gene; Multiplex-PCR; Integron

MeSH Terms

Ampicillin
Anti-Bacterial Agents
Bacteriophage Typing
Chloramphenicol
Diffusion
Disease Outbreaks
Drug Resistance, Microbial
Humans
Integrons
Kanamycin
R Factors
Salmonella enterica*
Salmonella*
Seoul*
Streptomycin
Tetracycline
Trimethoprim
Ampicillin
Anti-Bacterial Agents
Chloramphenicol
Kanamycin
Streptomycin
Tetracycline
Trimethoprim