J Breast Cancer.  2005 Jun;8(2):45-51. 10.4048/jbc.2005.8.2.45.

Expression of estrogen receptor beta, estrogen receptor alpha and cyclooxygenase II in advanced breast cancer

• Baek JM
• Sung GY
• Lee DS
• Chun KH
• Lee DH
• Seo YJ
• Choi SH
• Park WC
• Kim JS
• Song BJ
• Oh SJ
• Kim W
• Park IY
• Jung SS
• Lim KW
• Won JM
• Chun CS
• Lee JH
• Kim JA

• KMID: 1573949
• DOI: http://doi.org/10.4048/jbc.2005.8.2.45

Abstract

PURPOSE
Although the role of the estrogen receptor alpha (ER alpha, previously called the estrogen receptor) in breast cancer is well established, that of the second human estrogen receptor (ER), estrogen receptor beta (ER beta), remains uncertain. The expression of cyclooxygenase II (COX II) could also be regulated by sex steroids such as estrogen and progesterone. To investigate whether the expressions of the ER beta, ER alpha, and COX II are elevated in more aggressive breast cancers, the expression of the ER beta was studied by immunohistochemical staining in 20 primary breast cancer and original breast cancer tissues from 20 recurrent cancer patients, and its associations with ER alpha and cyclooxygenase (COX) II were evaluated. METHODS: Paraffin tissue sections from 40 breast cancers, surgically excised at the Department of Surgery, the Catholic University of Korea. were obtained. The immunohistochemical analysis was conducted on 20 non-recurrent, and 20 recurrent primary breast cancer tissues, using polyclonal antibodies to ER beta, ER alpha, and the corresponding monoclonal antibodies to COX II. RESULTS: Of the 40 patients, 15 (37.5%) were ER beta-positive, 30 (75%) were ER alpha-positive, and 24 (60%) were COX II-positive. The ER bata status was not related to the tumor size or menopausal status, but was related to the nodal status. The stati of ER alpha and COX II were not related to other clinico-pathological factors. The ER beta positivity was significantly more frequent in the study than the control group. (ER beta, p = 0.0222; ER alpha p = 0.1441; COX II, p = 1.00) The presence of ER beta was significantly related to the expression of ER alpha and COX II (p = 0.0455, p = 0.0381, respectively). CONCLUSION: These results suggest that the expression of ER beta is associated with early recurrence in breast cancer and the expression of COX II in the presence of ER beta implies the possibility of prognostic significance.

Keyword

breast neoplasms; estrogen receptors beta; estrogen receptors alpha; cyclooxygenase II; recurrence

MeSH Terms

Antibodies
Antibodies, Monoclonal
Breast Neoplasms*
Breast*
Estrogen Receptor alpha*
Estrogen Receptor beta*
Estrogens*
Humans
Korea
Paraffin
Progesterone
Prostaglandin-Endoperoxide Synthases*
Recurrence
Steroids
Antibodies
Antibodies, Monoclonal
Estrogen Receptor alpha
Estrogen Receptor beta
Estrogens
Paraffin
Progesterone
Prostaglandin-Endoperoxide Synthases
Steroids