Immune Netw.
2002 Sep;2(3):142-149. 10.4110/in.2002.2.3.142.
CD40-CD40 Ligand Interactions in the Production of IL-12 and IFN-gamma by Tuberculous Pleural Mononuclear Cells
- Affiliations
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- 1Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Korea. hayoungj@cnu.ac.kr
- 2Department of Internal Medicine, College of Medicine, Chungnam National University, Daejeon, Korea.
- 3Department of Internal Medicine, Catholic University, Daejeon, Korea.
- 4Department of Internal Medicine, College of Medicine, Konyang University, Chungnam, Korea.
- 5Department of Microbiology, College of Medicine, Konyang University, Chungnam, Korea.
- KMID: 1571516
- DOI: http://doi.org/10.4110/in.2002.2.3.142
Abstract
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BACKGROUND: Our previous study showed that purified protein derivative (PPD)- stimulated pleural mononuclear cells (PMC) from tuberculous pleurisy (Tbp) produced significantly more IFN-gamma (10- to 70-fold) after in vitro PPD stimulation than freshly isolated pleural cells from malignant pleurisy. The present study was designed to determine whether blocking the CD40-CD40 ligand (CD40L) interaction decreases IFN-gamma production by altering IL-12 levels.
METHODS
IL-12 and IFN-gamma production after neutralizing anti-CD40L antibody treatment was compared to the efficacy of anti-CD80, anti-CD86, and a combination of anti-CD80 and CD86 (CD80+86) monoclonal antibodies (mAb). These activities were measured by enzyme-linked immunosorbent assays (ELISAs) and reverse transcription-polymerase chain reaction (RT-PCR), after in vitro stimulation with PPD antigen (Ag).
RESULTS
Neutralization of CD80, CD86 and CD80+86 did not decrease IFN-gamma and IL-12 production in Tbp-PMC, whereas neutralization of CD40L significantly depressed IL-12 p40 and IFN-gamma. In addition, neutralization of CD40L completely inhibited IL-12 p40 and IFN-gamma mRNA expression.
CONCLUSION
The CD40-CD40L interaction might play a major role in IL-12 and IFN-gamma production in Tbp-PMC, thus contributing to protective immunity in human tuberculosis.
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