Immune Netw.  2002 Sep;2(3):133-136. 10.4110/in.2002.2.3.133.

Alloimmune and Skin Allograft Responses in 4-1BB (CD137)-deficient Mice

• Wolisi G
• Srirangam
• Vinay DS
• Suh JH
• Suh HS
• Choi BK
• Kwon BS

• KMID: 1571514
• DOI: http://doi.org/10.4110/in.2002.2.3.133

Abstract

BACKGROUND: The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death.
METHODS
In this study, female C57BL/6 (H-2(b)) mice were used as a recipient, and DBA/2 (H-2(d)) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA.
RESULTS
Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB- deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 (H-2(b)) mice were grafted with the trunk skin of DBA/2 (H-2d) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates.
CONCLUSION
These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.

Keyword

4-1BB; skin graft; alloimmunity; MLR

MeSH Terms

Allografts*
Animals
Cell Proliferation
Cell Survival
Enzyme-Linked Immunosorbent Assay
Female
Graft Survival
Hand
Humans
Interleukin-2
Lymphocyte Culture Test, Mixed
Mice*
Necrosis
Nerve Growth Factor
Skin*
Tissue Donors
Transplants
Interleukin-2
Nerve Growth Factor