Korean J Immunol.  1999 Jun;21(2):175-181.

Effects of Korean Traditional Medicine on Murine Hematopoiesis (2) - Single Transduction by Korean Traditional Medicine -

• Kim SH
• Lim JS

Abstract

Recently, we found that the Korean traditional medicine could enhance the expression of some cytokine in murine bone marrow cell culture. To understand the mode of actions of the traditional medicine, we examined the pattern of protein phosphorylation in bone marrow cells treated with Korean traditional medicine. When bone marrow cells were treated with Korean traditional medicine, the phosphorylation of p45, p50, p84/p91 kinase was observed, and gene expression of TPO and SCF was related to p45 and p91. It had been reported that TPO, SCF, IL-3 and GM-CSF gene expression transmits signal through the JAK-STAT pathway. Molecular involvement of JAK-STAT in signal induced by Korean traditional medicine was analyzed by anti-JAK and anti-STAT-1 Ab. To analyze the transcriptional factor that promotes the cytokine gene expression by the stirnulation of Korean traditional medicine, GAS and NF-B binding activity was analyzed by EMSA. We found the increase in NF-B at SMT and BHT. Therefore, we conclude that the cytokine gene expression induced by Korean traditional medicine was mediated by the JAK-STAT pathway and by the combination of GAS and NF-kB.

Keyword

Traditional Medicine; Hematopoiesis; Signal Transduction; Cytokine

MeSH Terms

Bone Marrow Cells
Butylated Hydroxytoluene
Gene Expression
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoiesis*
Interleukin-3
Medicine, Korean Traditional*
Medicine, Traditional
NF-kappa B
Phosphorylation
Phosphotransferases
Signal Transduction
Butylated Hydroxytoluene
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-3
NF-kappa B
Phosphotransferases