Biomol Ther.  2013 Mar;21(2):121-125.

Hesperetin Stimulates Cholecystokinin Secretion in Enteroendocrine STC-1 Cells

• Kim HY
• Park M
• Kim K
• Lee YM
• Rhyu MR

Abstract

Hesperetin (3',5,7-trihydroxy 4'-methoxyflavanone) and its glycoside hesperidin (hesperetin 7-rhamnoglucoside) in oranges have been reported to possess pharmacological effects related to anti-obesity. However, hesperetin and hesperidin have not been studied on suppressive effects on appetite. This study examined that hesperetin and hesperidin can stimulate the release of cholecystokinin (CCK), one of appetite-regulating hormones, from the enteroendocrine STC-1 cells, and then examined the mechanisms involved in the CCK release. Hesperetin significantly and dose-dependently stimulated CCK secretion with an EC50 of 0.050 mM and increased the intracellular Ca2+ concentrations ([Ca2+]i) compared to the untreated control. The stimulatory effect by hesperetin was mediated via the entry of extracellular Ca2+ and the activation of TRP channels including TRPA1. These results suggest that hesperetin can be a candidate biomolecule for the suppression of appetite and eventually for the therapeutics of obesity.

Keyword

Hesperetin; Cholecystokinin; Intracellular Ca2+; TRP ankyrin 1; Enteroendocrine cells

MeSH Terms

Appetite
Cholecystokinin*
Citrus sinensis
Enteroendocrine Cells
Hesperidin
Obesity
Cholecystokinin
Hesperidin