Clin Exp Reprod Med.
2013 Jun;40(2):67-75. 10.5653/cerm.2013.40.2.67.
Peroxisome proliferator-activated receptor gamma agonist suppresses human telomerase reverse transcriptase expression and aromatase activity in eutopic endometrial stromal cells from endometriosis
- Affiliations
-
- 1Health Promotion Center, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.
- 2Department of Obstetrics and Gynecology, Ajou University School of Medicine, Suwon, Korea. kjhwang@ajou.ac.kr
- 3Department of Molecular Science and Technology, Ajou University, Suwon, Korea.
- 4Department of Obstetrics and Gynecology, Bundang Jesaeng Hospital, Seongnam, Korea.
- KMID: 1493212
- DOI: http://doi.org/10.5653/cerm.2013.40.2.67
Abstract
OBJECTIVE
To investigate the effect of peroxisome proliferator activated receptor gamma (PPARgamma) agonist on the cell proliferation properties and expression of human telomerase reverse transcriptase (hTERT) and aromatase in cultured endometrial stromal cell (ESC) from patients with endometriosis.
METHODS
Human endometrial tissues were obtained from women with endometriosis and healthy women (controls) using endometrial biopsy. Isolated ESCs were cultured and the cell proliferation was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and expression of hTERT, aromatase, and cyclooxygenase (COX)-2 by western blotting according to the addition of rosiglitazone (PPARgamma agonist).
RESULTS
We demonstrate that the cultured ESCs of endometriosis showed hTERT protein overexpression and increased cellular proliferation, which was inhibited by rosiglitazone, in a dose-dependent manner. At the same time, PPARgamma agonist also inhibited aromatase and COX-2 expression, resulting in decreased prostaglandin E2 production in the ESCs of endometriosis.
CONCLUSION
This study suggests that PPARgamma agonist plays an inhibitory role in the proliferative properties of eutopic endometrium with endometriosis by down-regulation of hTERT and COX-2 expression; this could be a new treatment target for endometriosis.
Keyword
MeSH Terms
-
Aromatase
Biopsy
Biphenyl Compounds
Blotting, Western
Cell Proliferation
Dinoprostone
Down-Regulation
Endometriosis
Endometrium
Female
Humans
Peroxisomes
PPAR gamma
Prostaglandin-Endoperoxide Synthases
Stromal Cells
Telomerase
Thiazolidinediones
Aromatase
Biphenyl Compounds
Dinoprostone
PPAR gamma
Prostaglandin-Endoperoxide Synthases
Telomerase
Thiazolidinediones
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Gonadotropin-releasing hormone agonist treatments reduce aromatase and cyclooxygenase-2 in ovarian endometrioma and eutopic endometium in women with endometriosis
- Effect of Transforming Growth Factor (TGF)-beta and Peroxisome Proliferator-Activated Receptor (PPAR)-gamma in Endometrial Decidualization
- Role of ERK (Extracellular Signal Regulated Kinas) and PPAR gamma(Peroxisome Proliferator-Activated Receptor Gamma) on TGF-beta1 Induced Human Endometrial Stromal Cell Decidualization
- The Effect of Chlamydia pneumoniae on the Expression of Peroxisome Proliferator-Activated Receptor-gamma in Vascular Smooth Muscle Cells
- Expression of EGF mRNA and protein in eutopic and ectopic endometrial tissues of patients with endometriosis
