Korean J Fertil Steril.  2006 Jun;33(2):105-113.

Role of ERK (Extracellular Signal Regulated Kinas) and PPAR gamma(Peroxisome Proliferator-Activated Receptor Gamma) on TGF-beta1 Induced Human Endometrial Stromal Cell Decidualization

• Chang HJ
• Lee JH
• Kim MR
• Hwang KJ
• Park DW
• Min CK

Abstract

OBJECTIVE
To investigate the role of ERK and PPAR gamma on the TGF-beta1 induced human endometrial stromal cell decidualization in vitro. METHOD: Endometrial stromal cells are cultured under the following condition: DMEM/F12 (10% FBS, 1 nM E2 and 100 nM P4). TGF-beta1 (5 ng/ml), Rosiglitazone (50 nM), and PD98059 (20 microgram) were added according to experimental purposes. Trypan-Blue and hematocytometer were utilized to count cell number. Enzyme-linked immunosorbent assay (ELISA) and western blotting were utilized to detect proteins. RESULT: TGF-beta1 inhibited proliferation of cultured human endometrial stromal cells and induced expression of PGE2 and prolactin. This effect was mediated by Smad and ERK activation. Administration of rosiglitazone, PPAR gamma agonist, prevented TGF-beta1 effect on cell proliferation. Furthermore, Rosiglitazone inhibited TGF-beta1 induced activation of ERK, consequently reduced PGE2 and prolactin production.
CONCLUSION
TGF-beta1 induced decidualization of endometrial stromal cell through Smad and ERK phosphorylation. PPAR gamma acts as a negative regulator of human endometrial cell decidualization in vitro.

Keyword

Decidualization; TGF-beta1; PPAR gamma; ERK; Smad

MeSH Terms

Blotting, Western
Cell Count
Cell Proliferation
Dinoprostone
Enzyme-Linked Immunosorbent Assay
Humans*
Peroxisome Proliferator-Activated Receptors*
Phosphorylation
PPAR gamma
Prolactin
Stromal Cells*
Transforming Growth Factor beta1*
Dinoprostone
PPAR gamma
Peroxisome Proliferator-Activated Receptors
Prolactin
Transforming Growth Factor beta1