Mycobiology.  2008 Sep;36(3):173-177. 10.4489/MYCO.2008.36.3.173.

Antishigellosis and Cytotoxic Potency of Crude Extracts and Isolated Constituents from Duranta repens

• Nikkon F
• Habib MR
• Karim MR
• Hossain MS
• Mosaddik M
• Haque ME

• KMID: 1487339
• DOI: http://doi.org/10.4489/MYCO.2008.36.3.173

Abstract

The crude ethanol extracts (stem and fruits), their fractions and two triterpenes, beta-Amyrin and 12-Oleanene 3beta, 21beta-diol, isolated as a mixture from the chloroform soluble fraction of an ethanolic extract of Duranta repens stem, were evaluated for antibacterial, antifungal activities by the disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The structures of the two compounds were confirmed by IR, 1H-NMR, 13C-NMR and LC-MS spectral data. The chloroform soluble fraction of stem and ethanol extract of fruits possess potent antishigellosis activity and also exhibited moderate activity against some pathogenic bacteria and fungi but the isolated compound 1 (mixture of beta-Amyrin and 12-Oleanene 3beta, 21beta-diol) showed mild to moderate inhibitory activity to microbial growth. The minimum inhibitory concentrations (MICs) of the extracts (stem and fruits), their fractions and compound 1 were found to be in the range of 32~128 microg/ml. The chloroform soluble fractions of stem and ethanol extract of fruit showed significant cytotoxicity with LC50 value of 0.94 microg/ml and 0.49 microg/ml, respectively against brine shrimp larvae.

Keyword

beta-Amyrin; Antimicrobial activity; Cytotoxicity; Duranta repens; 12-Oleanene-3-beta; 21-beta-diol

MeSH Terms

Artemia
Bacteria
Biological Assay
Chloroform
Complex Mixtures
Diffusion
Ethanol
Fruit
Fungi
Larva
Microbial Sensitivity Tests
Oleanolic Acid
Salts
Triterpenes
Chloroform
Complex Mixtures
Ethanol
Oleanolic Acid
Salts
Triterpenes