J Korean Ophthalmol Soc.  2008 Jul;49(7):1154-1158. 10.3341/jkos.2008.49.7.1154.

Effects of Intravitreal Amniotic Membrane Injection in a Rat Model of Choroidal Neovascularization

• Lee JH
• Chung JL
• Hong S
• Koh HJ

• KMID: 1476971
• DOI: http://doi.org/10.3341/jkos.2008.49.7.1154

Abstract

PURPOSE: To evaluate the effects of an intravitreal amniotic membrane (AM) injection on a rat model of laser-induced choroidal neovascularization (CNV).
METHODS
Intravitreal injection of AM (12.5 microgram/mL) into the left eyes and phosphate-buffered saline into the fellow eyes of 15 rats was performed immediately after laser injury. At week 2, after angiography, the rats were sacrificed, and histopathologic analysis was performed. Angiographic and histopathologic assessments of the effects of AM injection on CNV growth were compared between the two groups.
RESULTS
CNV thickness was found to have increased more in the AM treatment group (141.54+/-8.12 micrometer) than in the experimental control group (65.23+/-16.90 micrometer) (p=0.010).
CONCLUSIONS
Intravitreal AM injection demonstrated histopathologic pro-angiogenic properties in a rat model of CNV.

Keyword

Amniotic membrane; Angiogenesis; Choroidal neovascularization

MeSH Terms

Amnion
Angiography
Animals
Choroid
Choroidal Neovascularization
Eye
Intravitreal Injections
Rats

Figure

• 

  Figure 1. Histopathologic sections in hematoxylin-eosin (H&E) staining showing choroidal neovascularization induced by laser. (A) Amniotic membrane treatment group; (B) Experimental control group (400 times magnification, high power field view, light microscope).

Reference

References

1. Lu M, Adamis AP. Molecular biology of choroidal neovascularization. Ophthalmol Clin North Am. 2006; 19:323–34.

2. Sun JK, Miller JW. Medical treatment of choroidal neovascularization secondary to age-related macular degeneration. Int Ophthalmol Clin. 2005; 45:115–32.

3. Rakic JM, Lambert V, Munaut C. . Mice without uPA, tPA, or plasminogen genes are resistant to experimental choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003; 44:1732–9.

4. Heiligenhaus A, Li HF, Yang Y. . Transplantation of amniotic membrane in murine herpes stromal keratitis modulates matrix metalloproteinases in the cornea. Invest Ophthalmol Vis Sci. 2005; 46:4079–85.

5. Lee JH, Lee HK, Kim JK. . Expression of laminin-5 with amniotic membrane transplantation in excimer laser ablated rat corneas. J Cataract Refract Surg. 2004; 30:2192–9.

6. Saw VP, Minassian D, Dart J. . Amniotic membrane transplantation for ocular disease: a review of the first 233 cases from the UK user group. Br J Ophthalmol. 2007; 91:1042–7.

7. Shao C, Sima J, Zhang SX. . Suppression of corneal neovascularization by PEDF release from human amniotic membranes. Invest Ophthalmol Vis Sci. 2004; 45:1758–62.

8. Takahashi H, Igarashi T, Fujimoto C. . Immunohistochemical observation of amniotic membrane patching on a corneal alkali burn in vivo. Jpn J Ophthalmol. 2007; 51:3–9.

9. Kiilgaard JF, Prause JU, Prause M. . Subretinal posterior pole injury induces selective proliferation of RPE cells in the periphery in in vivo studies in pigs. Invest Ophthalmol Vis Sci. 2007; 48:355–60.

10. Lassota N, Kiilgaard JF, Prause JU, la Cour M. Correlation between clinical and histological features in a pig model of choroidal neovascularization. Graefes Arch Clin Exp Ophthalmol. 2006; 244:394–8.

11. Lee MS, Lee KM, Kim SH, Kwon OW. Adhesion between amniotic membrane and retinal tissue and inhibition of amniotic membrane on cell transformation. J Korean Ophthalmol Soc. 2003; 44:459–71.

12. Koh HJ, Bessho K, Cheng L. . Inhibition of choroidal neovascularization in rats by the urokinase-derived peptide A6. Invest Ophthalmol Vis Sci. 2004; 45:635–40.

13. Campochiaro PA. Ocular neovascularisation and excessive vascular permeability. Expert Opin Biol Ther. 2004; 4:1395–402.

14. Kobayashi N, Kabuyama Y, Sasaki S. . Suppression of corneal neovascularization by culture supernatant of human amniotic cells. Cornea. 2002; 21:62–7.

15. Ha HS, Song KY, Kim JC. Ultrastructural Analysis of in vivo Expanded Corneal Epithelium on Amniotic Membrane. J Korean Med Sci. 2006; 21:544–9.

16. Kim MK, Lee JL, Wee WR. Lee JH. Adhesion complex in cultivated limbal epithelium on amniotic membrane after transplantation into chemical burn model. J Korean Ophthalmol Soc. 2004; 45:1731–41.

17. Park GS, Je J, Kim JC. Stepwise surgical approach for in vivo expansion of epithelial stem cells to treating severe acute chemical burns with total limbal deficiency. Korean J Ophthalmol. 2003; 17:75–82.

18. Alviano F, Fossati V, Marchionni C. . Term Amniotic membrane is a high throughput source for multipotent Mesenchymal Stem Cells with the ability to differentiate into endothelial cells in vitro. BMC Dev Biol. 2007; 7:11.

19. Cheung CY, Brace RA. Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes. J Soc Gynecol Investig. 1999; 6:179–85.