Korean J Clin Microbiol.  2010 Dec;13(4):151-156. 10.5145/KJCM.2010.13.4.151.

Comparison of BACTEC Plus Aerobic/F Media and BacT/Alert FA Media to Detect Bacteria in Blood Culture Bottles Containing Peak Therapeutic Levels of Antimicrobials

Affiliations
  • 1Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea. miae@ewha.ac.kr

Abstract

BACKGROUND
Blood culture bottles with an antimicrobial removal system have been developed for patients treated with antibiotics. This study compared the ability of BACTEC Plus Aerobic/F bottles (Becton Dickinson, USA, BACTEC Plus) and BacT/Alert FA bottles (bio-Merieux Vitek, France) to effectively remove antimicrobials.
METHODS
BACTEC Plus and BacT/Alert FA bottles were spiked with 5 mL human blood, peak therapeutic concentrations of 9 antimicrobials and 7 type strains. Three rounds of duplicate testing were completed per antimicrobial/strain combination and growth control without antimicrobials. The time to detection (TTD) and recovery rates for bacteria were compared for both systems.
RESULTS
Overall, the BACTEC Plus and BacT/Alert FA recovered 76% (128/168) and 34% (57/168) of strains from test bottles, respectively. BACTEC Plus detected all of gram-positive bacteria except S. pneumoniae with ampicillin and ceftriaxone, but BacT/Alert FA detected 0~50% of gram-positive bacteria except E. faecalis with vancomycin and methicillin-resistant S. aureus with oxacillin. In presence of cefepime, cefotaxime, cefoxitin and ceftriaxone, BACTEC Plus detected 33~100% of gram-negative bacteria, but BacT/ Alert FA did not detect gram-negative bacteria at all. In presence of ciprofloxacin, BacT/Alert FA detected 100% of E. coli and K. pneumoniae compared with 33% of those for BACTEC Plus. Overall, TTD of BACTEC Plus was shorter than that of BacT/Alert FA except in detecting gram-negative bacteria with ciprofloxacin (P<0.05).
CONCLUSION
BACTEC Plus Aerobic/F media containing peak therapeutic levels of antimicrobials are more effective and faster detection of bacteria than BacT/Alert FA media.

Keyword

Blood culture; Antimicrobial removal system; BACTEC Plus Aerobic/F; BacT/Alert FA

MeSH Terms

Ampicillin
Anti-Bacterial Agents
Bacteria
Cefotaxime
Cefoxitin
Ceftriaxone
Cephalosporins
Ciprofloxacin
Gram-Negative Bacteria
Gram-Positive Bacteria
Humans
Methicillin Resistance
Oxacillin
Pneumonia
Vancomycin
Ampicillin
Anti-Bacterial Agents
Cefotaxime
Cefoxitin
Ceftriaxone
Cephalosporins
Ciprofloxacin
Oxacillin
Vancomycin

Figure

  • Fig. 1. Overall recovery rates from growth controls and test bottles for the BACTEC Plus and BacT/Alert FA system.

  • Fig. 2. Overall recovery rates of organisms in BACTEC Plus Aerobic/F and BacT/Alert FA bottles containing antimicrobials.


Cited by  2 articles

Development of Blood Culture and Quality Improvement
Sunjoo Kim
Ann Clin Microbiol. 2013;16(4):153-161.    doi: 10.5145/ACM.2013.16.4.153.

Comparison of the BACTEC Peds Plus Pediatric Blood Culture Bottle to the BacT/Alert PF Pediatric Blood Culture Bottle for Culturing Blood from Pediatric Patients
Kon-Hee Lee, Wonkeun Song, Min-Jeong Park, Jeongwon Hyun, Hyun Soo Kim, Kyu Man Lee
Korean J Clin Microbiol. 2011;14(4):126-130.    doi: 10.5145/KJCM.2011.14.4.126.


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