J Korean Soc Ther Radiol Oncol.  2010 Dec;28(4):211-218. 10.3857/jkstro.2010.28.4.211.

Catalase Induced by All-Trans Retinoic Acid Is Involved in Antiproliferation of 36B10 Cells

• Park WY
• Yu JR

• KMID: 1460138
• DOI: http://doi.org/10.3857/jkstro.2010.28.4.211

Abstract

PURPOSE
All-trans retinoic acid (ATRA) has antiproliferative effects against brain tumor cells. Recently, ATRA has been reported to induce catalase. We investigated whether catalase induction by ATRA is associated with its antiproliferative effects.
MATERIALS AND METHODS
36B10 cells were exposed to 0~50microM ATRA for 24 or 48 hours and mRNA, protein, and activity of catalase were measured. Reactive oxygen species (ROS) were measured using 2',7'-dichlorofluorescin diacetate. A clonogenic assay was used to confirm the cytotoxic effect.
RESULTS
The mRNA, protein, and activity of catalase were found to increase in a concentration- and incubation-time-dependent manner. The increase in catalase activity induced by ATRA was decreased by the addition of 3-amino-1,2,4-triazole (ATZ). ROS was also increased with ATRA and decreased by the addition of ATZ. The decrease in cell survival induced by ATRA was partly rescued by ATZ.
CONCLUSION
Catalase induction by ATRA is involved in ROS overproduction and thus inhibits the proliferation of 36B10 cells.

Keyword

All-trans retinoic acid; Catalase; Reactive oxygen species

MeSH Terms

Amitrole
Brain Neoplasms
Catalase
Cell Survival
Fluoresceins
Reactive Oxygen Species
RNA, Messenger
Tretinoin
Amitrole
Catalase
Fluoresceins
RNA, Messenger
Reactive Oxygen Species
Tretinoin