Anat Cell Biol.  2011 Dec;44(4):245-255. 10.5115/acb.2011.44.4.245.

Induced pluripotent stem cells and personalized medicine: current progress and future perspectives

• Chun YS
• Byun K
• Lee B

• KMID: 1447438
• DOI: http://doi.org/10.5115/acb.2011.44.4.245

Abstract

Generation of induced pluripotent stem cells (iPSCs) has revolutionized the field of regenerative medicine by providing researchers with a unique tool to derive disease-specific stem cells for study. iPSCs can self-renew and can differentiate into many cell types, offering a potentially unlimited source of cells for targeted differentiation into somatic effector cells. Hence, iPSCs are likely to be invaluable for therapeutic applications and disease-related research. In this review, we summarize the recent progress of iPSC generation that has been made with an emphasis on both basic and clinical applications including disease modeling, drug toxicity screening/drug discovery and cell replacement therapy.

Keyword

Induced pluripotent stem cell; Disease modeling; Genomics; Proteomics

MeSH Terms

Drug Design
Genomics
Humans
Induced Pluripotent Stem Cells
Proteomics
Regenerative Medicine
Stem Cells

Figure

• 

  Fig. 1 Generation of isogenic pairs of wild type and mutant induced pluripotent stem cells (iPSCs) using zinc finger nucleases for correcting a target sequence. When the DNA-binding and DNA-cleaving domains are fused together a highly specific pair of 'genomic scissors' is created, which binds with 24-36 bp specificity of the zinc finger nucleases (ZFNs) and cleaves the DNA of iPSCs. Homology directed repair with normal donor DNA can be applied to the DNA cleavage site of iPSCs. Gene edited iPSCs can be differentiated into lineage specific way such as cardiomyocyte, neuron or hepatocyte etc.

• 

  Fig. 2 Potential applications of human induced pluripotent stem cells (iPSCs). iPSC technology can be potentially utilized in disease modeling, drug discovery, gene therapy, and cell replacement therapy. Cell replacement therapy with healthy iPSC-derived cells is also a possible future development. Genetic mutations can be targeted by gene therapy approaches before or after reprogramming.

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