Int J Stem Cells.  2021 May;14(2):127-137. 10.15283/ijsc20143.

Vascularized Organoids: A More Complete Model

• Yu J

• KMID: 2515990
• DOI: http://doi.org/10.15283/ijsc20143

Abstract

As an emerging research model in vitro, organoids have achieved major progress in recapitulating morphological aspects of organs and personalized precision therapy. Various organoids have been currently constructed in vitro (e.g., brain, heart, liver, and gastrointestinal). Though there are prominent advantages on microstructures and partial functions, most of them have been encountering a frustrating challenge that stromal components (e.g., blood vessels) are in short supplement, which has imposed the main dilemma on the application of such model ex vivo. As advanced technologies, co-culturing pluripotent stem cells, mesenchymal stem cells, with endothelial cells on 3D substrate matrix, are leaping forward, a novel model of an organoid with vascularization is formed. The mentioned contribute to the construction of the functional organoids derived from corresponding tissues, making them more reliable in stem cell research and clinical medicine. The present study overall summarizes progress of the evolution, applications and prospects of vascularized organoids.

Keyword

Vascular organoids; Vascular organoids; Pluripotent stem cells; Pluripotent stem cells; Translational medicine; Translational medicine; Applications; Applications

Figure

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  Fig. 1 Organoids have been established of different systems.

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  Fig. 2 The procedures of vascular organoids. Co-culturing with embryonic or adult stem cells, endothelial cells and mesenchymal cells, vascular organoids are generated after transplantation (top); On some occasions where there are urgent for the vascular networks, it can be constituded well ex vivo following the steps (medium); As for diseases, blood vessels organoids are masterpieces, in addition to some cytokines and small molecules to form the stem cells niche, it can be generated (bottom).

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  Fig. 3 The application prospects of vascular organoids.

Reference

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