Tissue Eng Regen Med.  2024 Jul;21(5):653-671. 10.1007/s13770-024-00628-2.

Lung Organoids: Systematic Review of Recent Advancements and its Future Perspectives

• Thangam T
• Parthasarathy K
• Supraja K
• Haribalaji V
• Sounderrajan V
• Rao SS
• Jayaraj S

• KMID: 2557863
• DOI: http://doi.org/10.1007/s13770-024-00628-2

Abstract

Organoids are essentially an in vitro (lab-grown) three-dimensional tissue culture system model that meticulously replicates the structure and physiology of human organs. A few of the present applications of organoids are in the basic biological research area, molecular medicine and pharmaceutical drug testing. Organoids are crucial in connecting the gap between animal models and human clinical trials during the drug discovery process, which significantly lowers the time duration and cost associated with each stage of testing. Likewise, they can be used to understand cell-to-cell interactions, a crucial aspect of tissue biology and regeneration, and to model disease pathogenesis at various stages of the disease. Lung organoids can be utilized to explore numerous pathophysiological activities of a lung since they share similarities with its function. Researchers have been trying to recreate the complex nature of the lung by developing various ‘‘Lung organoids’’ models. This article is a systematic review of various developments of lung organoids and their potential progenitors. It also covers the in-depth applications of lung organoids for the advancement of translational research. The review discusses the methodologies to establish different types of lung organoids for studying the regenerative capability of the respiratory system and comprehending various respiratory diseases. Respiratory diseases are among the most common worldwide, and the growing burden must be addressed instantaneously. Lung organoids along with diverse bio-engineering tools and technologies will serve as a novel model for studying the pathophysiology of various respiratory diseases and for drug screening purposes.

Keyword

Lung organoids; Stem cells; Lung progenitors; Respiratory disorder; In vitro human model