Int J Stem Cells.  2024 Feb;17(1):38-50. 10.15283/ijsc23146.

Unleashing the Power of Undifferentiated Induced Pluripotent Stem Cell Bioprinting: Current Progress and Future Prospects

• Kim B
• Kim J
• Lee S

• KMID: 2552016
• DOI: http://doi.org/10.15283/ijsc23146

Abstract

Induced pluripotent stem cell (iPSC) technology has revolutionized various fields, including stem cell research, disease modeling, and regenerative medicine. The evolution of iPSC-based models has transitioned from conventional two-dimensional systems to more physiologically relevant three-dimensional (3D) models such as spheroids and organoids. Nonetheless, there still remain challenges including limitations in creating complex 3D tissue geometry and structures, the emergence of necrotic core in existing 3D models, and limited scalability and reproducibility. 3D bioprinting has emerged as a revolutionary technology that can facilitate the development of complex 3D tissues and organs with high scalability and reproducibility. This innovative approach has the potential to effectively bridge the gap between conventional iPSC models and complex 3D tissues in vivo. This review focuses on current trends and advancements in the bioprinting of iPSCs. Specifically, it covers the fundamental concepts and techniques of bioprinting and bioink design, reviews recent progress in iPSC bioprinting research with a specific focus on bioprinting undifferentiated iPSCs, and concludes by discussing existing limitations and future prospects.

Keyword

Induced pluripotent stem cells; 3D bioprinting; Tissue engineering

Figure

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  Fig. 1 Sequential evolution of induced pluripotent stem cell (iPSC)-based in vitro models depicting transition from 2D culture to 3D organoids, and further to 3D bioprinting. Schematics of 2D iPSC culture model (A), 3D organoid culture model (B), and 3D bioprinting culture model (C). Created with BioRender.com. ECM: extracellular matrix.

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  Fig. 2 Three major 3D bioprinting techniques used for induced pluripotent stem cell bioprinting. (A) Inkjet-based bioprinting, (B) extrusion-based bioprinting, and (C) laser-assisted bioprinting. Created with BioRender.com.

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  Fig. 3 Representation of bioink components used in 3D bio-printing. Created with BioRender.com.

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  Fig. 4 Induced pluripotent stem cell (iPSC)-based bioprinting strategies. Processes for pre-differentiated iPSC bioprinting (A) and undifferentiated iPSC bioprinting (B). Created with BioRender.com.

Reference

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