Lab Anim Res.  2012 Jun;28(2):91-97. 10.5625/lar.2012.28.2.91.

Synergistic anti-inflammatory effects of Laminaria japonica fucoidan and Cistanche tubulosa extract

• Kyung J
• Kim D
• Park D
• Yang YH
• Choi EK
• Lee SP
• Kim TS
• Lee YB
• Kim YB

• KMID: 1436701
• DOI: http://doi.org/10.5625/lar.2012.28.2.91

Abstract

The anti-inflammatory effects of fuciodan and Cistanche tubulosa (CT) extract were investigated in vitro macrophage culture system and in vivo carrageenan-induced air pouch inflammation model. CT extract inhibited nitric oxide production from activated RAW 264.7 macrophage cells, while fucoidan was inactive. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of fucoidan or CT extract. Moreover, tissue inflammation was substantially attenuated by the combinational therapy. However, there was no synergistic effect against the inflammatory cell infiltration, although fucoidan and CT extract each markedly reduced the cell numbers. Therefore, it is suggested that fucoidan blocks infiltration of inflammatory cells, while CT extract inhibits activation of the cells, and that their combinational treatment could be a promising candidate for the relief of various types of inflammation.

Keyword

Carrageenan; inflammation; fucoidan; Cistanche tubulosa extract

MeSH Terms

Carrageenan
Cell Count
Cistanche
Dinoprostone
Exudates and Transudates
Inflammation
Laminaria
Macrophages
Nitric Oxide
Polysaccharides
Carrageenan
Dinoprostone
Nitric Oxide
Polysaccharides

Figure

• 

  Figure 1 Effects of fucoidan (A) and Cistanche tubulosa (CT) extract (B) on the nitric oxide production of RAW 264.7 cells stimulated with interferon-γ (IFN-γ, 10 U/mL) and lipopolysaccharide (LPS, 10 µg/mL). *Significantly different from vehicle control (P<0.05). #Significantly different from IFN-γ+LPS alone (P<0.05).

• 

  Figure 2 Effects of fucoidan and Cistanche tubulosa (CT) extract (in mg/kg) on the carrageenan-induced exudation and inflammatory responses in an air-pouch inflammation model. A: net exudate volume 6 hours after carrageenan injection. B: nitric oxide (NO) concentration in the exudates. C: prostaglandin E2 (PGE2) concentration in the exudates. *Significantly different from normal (Nor) control (P<0.05). #Significantly different from carrageenan (Car) alone (P<0.05).

• 

  Figure 3 Representative microscopic findings of skin tissue of mice treated with carrageenan and/or fucoidan or Cistanche tubulosa (CT) extract. A, normal; B, carrageenan alone; C, fucoidan 18 mg/kg; D, fucoidan 54 mg/kg; E, CT extract 54 mg/kg; F, CT extract 162 mg/kg; G, fucoidan 18 mg/kg+CT extract 54 mg/kg; H, fucoidan 54 mg/kg+CT extract 162 mg/kg; I, fucoidan 90 mg/kg+CT extract 270 mg/kg. Bar=200 µm.

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