Lab Anim Res.  2015 Mar;31(1):24-32. 10.5625/lar.2015.31.1.24.

Effectiveness of the combinational treatment of Laminaria japonica and Cistanche tubulosa extracts in hair growth

• Shin K
• Kim TS
• Kyung J
• Kim D
• Park D
• Choi EK
• Lee SP
• Yang WS
• Kang MH
• Kim YB

• KMID: 1787648
• DOI: http://doi.org/10.5625/lar.2015.31.1.24

Abstract

Since scalp hair loss has increased recently even in young people, seriously affecting individual's quality of life, the hair growth-stimulating effects of Laminaria japonica extract (LJE) and Cistanche tubulosa extract (CTE) were investigated. After confirming anagen phase of follicles under shaving, male C57BL/6 mice were dermally applied with 3% Minoxidil or orally administered with the combinations of LJE and CTE for 21 days. Minoxidil promoted the hair regrowth and increased gamma-glutamyl transpeptidase (gamma-GTP) and alkaline phosphatase (ALP) activities. In addition, Minoxidil up-regulated epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) levels. Co-administration of LJE and CTE at 54 mg/kg LJE plus 162 mg/kg CTE exerted synergistic promoting effects on the hair regrowth, comparable to 3% Minoxidil. LJE preferentially enhanced ALP activity, while CTE increased both gamma-GTP and ALP activities as well as EGF and VEGF expressions. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of LJE and CTE. In addition, inflammatory cell infiltration was substantially inhibited by the combinational treatment. The results suggest that combinational oral treatment with LJE and CTE in appropriate doses and ratios prevent hair loss and improve alopecia, which might be in part mediated by their anti-inflammatory activities.

Keyword

Hair growth; inflammation; Laminaria japonica; Cistanche tubulosa; combinational treatment

MeSH Terms

Alkaline Phosphatase
Alopecia
Animals
Cistanche*
Dinoprostone
Epidermal Growth Factor
Exudates and Transudates
gamma-Glutamyltransferase
Hair*
Humans
Inflammation
Laminaria*
Male
Mice
Minoxidil
Nitric Oxide
Quality of Life
Scalp
Vascular Endothelial Growth Factor A
Alkaline Phosphatase
Dinoprostone
Epidermal Growth Factor
Minoxidil
Nitric Oxide
Vascular Endothelial Growth Factor A
gamma-Glutamyltransferase

Figure

• 

  Figure 1 Time-course of hair regrowth of mice treated with Minoxidil, or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE). ○, normal; •, Minoxidil (3%) application; ▵, 18 mg/kg LJE; ▾, 54 mg/kg LJE; □, 54 mg/kg CTE; ▪, 162 mg/kg CTE; ◇, 18 mg/kg LJE + 54 mg/kg CTE; ♦, 54 mg/kg LJE + 162 mg/kg CTE.

• 

  Figure 2 Representative gross findings of hair regrowth of mice treated with Minoxidil, or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) on day 21. A, normal; B, Minoxidil (3%) application; C, 18 mg/kg LJE; D, 54 mg/kg LJE; E, 54 mg/kg CTE; F, 162 mg/kg CTE; G, 18 mg/kg LJE+54 mg/kg CTE; H, 54 mg/kg LJE+162 mg/kg CTE.

• 

  Figure 3 Representative microscopic findings of hair regrowth of mice treated with Minoxidil, or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) on day 21. A, normal; B, Minoxidil (3%) application; C, 18 mg/kg LJE; D, 54 mg/kg LJE; E, 54 mg/kg CTE; F, 162 mg/kg CTE; G, 18 mg/kg LJE+54 mg/kg CTE; H, 54 mg/kg LJE+162 mg/kg CTE.

• 

  Figure 4 Effects of Minoxidil (MXD, 3%), or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) (in mg/kg) on γ-glutamyl transpeptidase (γ-GTP) activity on days 14 (A) and 21 (B) of treatment. *Significantly different from normal (Nor) control (P<0.05).

• 

  Figure 5 Effects of Minoxidil (MXD, 3%), or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) (in mg/kg) on alkaline phosphatase (ALP) activity on days 14 (A) and 21 (B) of treatment. *Significantly different from normal (Nor) control (P<0.05).

• 

  Figure 6 Effects of Minoxidil (MXD, 3%), or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) (in mg/kg) on epidermal growth factor (EGF) content on days 14 (A) and 21 (B) of treatment. *Significantly different from normal (Nor) control (P<0.05).

• 

  Figure 7 Effects of Minoxidil (MXD, 3%), or Laminaria japonica extract (LJE) and/or Cistanche tubulosa extract (CTE) (in mg/kg) on vascular endothelial growth factor (VEGF) content on days 14 (A) and 21 (B) of treatment. *Significantly different from normal (Nor) control (P<0.05).

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