Korean J Vet Res.  2012 Jun;52(2):99-104.

Differential gene expression pattern in brains of acrylamide-administered mice

Affiliations
  • 1Department of Toxicology & Biochemistry, College of veterinary Medicine, Jeju National University, Jeju 690-756, Korea. chhan@jejunu.ac.kr

Abstract

The present study was performed to evaluate the relationship between the neurotoxicity of acrylamide and the differential gene expression pattern in mice. Both locomotor test and rota-rod test showed that the group treated with higher than 30 mg/kg/day of acrylamide caused impaired motor activity in mice. Based on cDNA microarray analysis of mouse brain, myelin basic protein gene, kinesin family member 5B gene, and fibroblast growth factor (FGF) 1 and its receptor genes were down-regulated by acrylamide. The genes are known to be essential for neurofilament synthesis, axonal transport, and neuro-protection, respectively. Interestingly, both FGF 1 and its receptor genes were down-regulated. Genes involved in nucleic acid binding such as AU RNA binding protein/enoyl-coA hydratase, translation initiation factor (TIF) 2 alpha kinase 4, activating transcription factor 2, and U2AF 1 related sequence 1 genes were down-regulated. More interesting finding was that genes of both catalytic and regulatory subunit of protein phosphatases which are important for signal transduction pathways were down-regulated. Here, we propose that acrylamide induces neurotoxicity by regulation of genes associated with neurofilament synthesis, axonal transport, neuro-protection, and signal transduction pathways.

Keyword

acrylamide; cDNA microarray; locomotor test; neurotoxicity; rota-rod test

MeSH Terms

Acrylamide
Activating Transcription Factor 2
Animals
Axonal Transport
Brain
Fibroblast Growth Factors
Gene Expression
Humans
Kinesin
Mice
Motor Activity
Myelin Basic Protein
Oligonucleotide Array Sequence Analysis
Peptide Initiation Factors
Phosphoprotein Phosphatases
Phosphotransferases
RNA
Signal Transduction
Acrylamide
Activating Transcription Factor 2
Fibroblast Growth Factors
Kinesin
Myelin Basic Protein
Peptide Initiation Factors
Phosphoprotein Phosphatases
Phosphotransferases
RNA
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