Korean J Radiol.  2013 Feb;14(1):30-37. 10.3348/kjr.2013.14.1.30.

In Vitro and In Vivo Imaging of Prostate Cancer Angiogenesis Using Anti-Vascular Endothelial Growth Factor Receptor 2 Antibody-Conjugated Quantum Dot

Affiliations
  • 1Medicinal Chemistry Laboratory, Institute Pasteur Korea (IP-K), Seongnam 463-400, Korea.
  • 2Department of Radiology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Institute of Radiation Medicine, Seoul National University Medical Research Center, Clinical Research Institute, Seongnam 463-707, Korea. h
  • 3Department of Nuclear Medicine and Biomedical Sciences Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea.

Abstract


OBJECTIVE
Authors aimed to determine the targeting ability of vascular endothelial growth factor receptor 2 (VEGFR2)-conjugated quantum dots (QDs) in vitro, and apply it for a xenograft prostate cancer mouse model.
MATERIALS AND METHODS
Conjugation reaction of QDs was performed by using the N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and sulfo-(N-hydroxysulfosuccinimide) (Sulfo-NHS). The human umbilical vein cord endothelial cells (HUVECs) were incubated with QDs, conjugated with antiVGFR2, to see a specific binding in vitro. Fluorescent cell images were taken by a confocal microscope. The human prostate cancer cells (PC3) were injected to five nude mice on hind limbs to make the xenograft tumor model. QD-antiVEGFR2 antibody complex was injected into the tumor model and fluorescence measurements were performed at 1, 4, 9, 12, 15, and 24 hours after the injection.
RESULTS
The specific interaction between HUVECs and QD-antiVEGFR2 antibody was clearly shown in vitro. The in vivo fluorescence image disclosed that there was an increased signal of tumor, 12 hours after the injection of QDs.
CONCLUSION
By showing endothelial cells binding with QDs-antiVEGFR2 antibodyand an experimental application of the antibody for VEGFR2 imaging in the prostate cancer xenograft mouse model, we suggests that the antibody-conjugated QDs can be a potential imaging tool for angiogenesis of the cancer.

Keyword

Quantum dot; VEGFR2; Angiogenesis; Prostate cancer; Near infrared

MeSH Terms

Animals
Carbodiimides/pharmacology
Cell Line, Tumor
Disease Models, Animal
Electrophoresis, Agar Gel
Fluorescence
Male
Mice
Mice, Nude
Microscopy, Confocal
Neovascularization, Pathologic/*pathology
Prostatic Neoplasms/*pathology
*Quantum Dots
Succinimides/pharmacology
Transplantation, Heterologous
Vascular Endothelial Growth Factor Receptor-2/*antagonists & inhibitors
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