J Korean Med Sci.  1992 Jun;7(2):147-153. 10.3346/jkms.1992.7.2.147.

A monoclonal antibody to common acute lymphoblastic leukemia antigen (CALLA) and its expression on several human tumor cell lines

• Park WS
• Hwang TS
• Kim TJ
• Park CS
• Bae YM
• Park SH
• Lee SK

• KMID: 1421905
• DOI: http://doi.org/10.3346/jkms.1992.7.2.147

Abstract

We describe a newly-made murine monoclonal antibody to the common acute lymphoblastic leukemia antigen (CALLA), named SHB-10. The antigen detected by SHB-10 has a molecular weight of about 105 kDa. This antibody is very similar to that of conventional anti-CD10 Ab on indirect flowcytometric analysis using lymphoid malignant cell lines and peripheral lymphocytes of acute lymphoblastic leukemia (ALL) patients. The binding of anti-CD10 to Daudi cell and peripheral lymphocytes of ALL patients is blocked by SHB-10. Thus this monoclonal antibody is thought to detect the CALLA. The distribution of antigen detected by SHB-10 on several cell lines of neuroectodermal tumor and lymphoid malignancy was analysed and a slight difference in their cell surface expression is observed when compared with that by conventional anti-CD10. Further biochemical analysis is now under way for a better characterization of this antigen.

Keyword

Common acute lymphoblastic leukemia antigen (CALLA); monoclonal antibody

MeSH Terms

Animals
Antibodies, Monoclonal/*immunology
Antigens, Differentiation/*analysis/immunology
Antigens, Neoplasm/*analysis/immunology
Flow Cytometry
Humans
Immunoglobulin Isotypes/analysis
Mice
Mice, Inbred BALB C
Neoplasms/*immunology
Neprilysin
Tumor Cells, Cultured
Tumor Markers, Biological/*analysis
Antibodies, Monoclonal
Antigens, Differentiation
Antigens, Neoplasm
Immunoglobulin Isotypes
Tumor Markers, Biological
Neprilysin