J Prev Med Public Health.  2012 Jul;45(4):251-258. 10.3961/jpmph.2012.45.4.251.

Quantitative Analysis of Cancer-associated Gene Methylation Connected to Risk Factors in Korean Colorectal Cancer Patients

Affiliations
  • 1Department of Preventive Medicine, Dong-A University College of Medicine, Busan, Korea. yshong@dau.ac.kr
  • 2Institute of Forensic Medicine, Pusan National University School of Medicine, Busan, Korea.
  • 3Department of Occupational Medicine, Kangbuk Samsung Hospital, Seoul, Korea.

Abstract


OBJECTIVES
The purpose of this paper was to elucidate the potential methylation levels of adjacent normal and cancer tissues by comparing them with normal colorectal tissues, and to describe the correlations between the methylation and clinical parameters in Korean colorectal cancer (CRC) patients.
METHODS
Hypermethylation profiles of nine genes (RASSF1, APC, p16INK4a, Twist1, E-cadherin, TIMP3, Smad4, COX2, and ABCB1) were examined with 100 sets of cancer tissues and 14 normal colorectal tissues. We determined the hypermethylation at a given level by a percent of methylation ratio value of 10 using quantitative methylation real-time polymerase chain reaction.
RESULTS
Nine genes' hypermethylation levels in Korean CRC patient tissues were increased more higher than normal colorectal tissues. However, the amounts of p16INK4a and E-cadherin gene hypermethylation in normal and CRC tissues were not significantly different nor did TIMP3 gene hypermethylation in adjacent normal and cancer tissues differ significantly. The hypermethylation of TIMP3, E-cadherin, ABCB1, and COX2 genes among other genes were abundantly found in normal colorectal tissues. The hypermethylation of nine genes' methylation in cancer tissues was not significantly associated with any clinical parameters. In Cohen's kappa test, it was moderately observed that RASSF1 was related with E-cadherin, and Smad4 with ABCB1 and COX2.
CONCLUSIONS
This study provides evidence for different hypermethylation patterns of cancer-associated genes in normal and CRC tissues, which may serve as useful information on CRC cancer progression.

Keyword

Hypermethylation; Colorectal neoplasms; Genetic markers

MeSH Terms

Colorectal Neoplasms/*genetics
*DNA Methylation
Disease Progression
*Gene Expression Profiling
Humans
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
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