Exp Mol Med.
2003 Aug;35(4):279-284.
Lipid rafts are important for the association of RANK and TRAF6
- Affiliations
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- 1National Research Laboratory for Bone Metabolism, Chosun University, Gwangju 501-759, Korea. jhblee@mail.chosun.ac.kr
- 2Research Center for Proteineous Materials.
- 3School of Dentistry, Chosun University, Gwangju 501-759, Korea.
- 4Department of Cell and Developmental Biology, College of Dentistry, Seoul National University, Seoul 110-749, Korea.
Abstract
- Rafts, cholesterol- and sphingolipid-rich membrane microdomains, have been shown to play an important role in immune cell activation. More recently rafts were implicated in the signal transduction by members of the TNF receptor (TNFR) family. In this study, we provide evidences that the raft microdomain has a crucial role in RANK (receptor activator of NF-kappaB) signaling. We found that the majority of the ectopically expressed RANK and substantial portion of endogenous TRAF2 and TRAF6 were detected in the low-density raft fractions. In addition, TRAF6 association with rafts was increased by RANKL stimulation. The disruption of rafts blocked the TRAF6 translocation by RANK ligand and impeded the interaction between RANK and TRAF6. Our observations demonstrate that proper RANK signaling requires the function of raft membrane microdomains.
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