J Vet Sci.  2008 Jun;9(2):127-131. 10.4142/jvs.2008.9.2.127.

Pyridoxine induced neuropathy by subcutaneous administration in dogs

• Chung JY
• Choi JH
• Hwang CY
• Youn HY

• KMID: 1106227
• DOI: http://doi.org/10.4142/jvs.2008.9.2.127

Abstract

To construct a sensory neuropathy model, excess pyridoxine (150 mg/kg s.i.d.) was injected subcutaneously in dogs over a period of 7 days. During the administrations period, the dogs experienced body weight reduction and proprioceptive loss involving the hindquarters. After pyridoxine administration was completed, electrophysiological recordings showed that the M wave remained at a normal state, but the H-reflex of the treated dogs disappeared at 7 days. The dorsal funiculus of L4 was disrupted irregularly in the axons and myelin with vacuolation. The dorsal root ganglia of L4, and sciatic and tibial nerves showed degenerative changes and vacuolation. However, the lateral and ventral funiculi of L4 showed a normal histopathologic pattern. Although this subcutaneous administration method did not cause systemic toxicity and effectively induced sensory neuropathy, this study confirmed the possibility of producing a pyridoxine-induced sensory neuropathy model in dogs with short-term administration.

Keyword

dog; H-reflex; pyridoxine; sensory neuropathy

MeSH Terms

Animals
Body Weight/drug effects
*Disease Models, Animal
Dogs
Electrophysiology
Neurodegenerative Diseases/*chemically induced/pathology
Proprioception/drug effects
Pyridoxine/*toxicity

Figure

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  Fig. 1 On electrophysiological analysis, a control dog showed an H-reflex (A), but a pyridoxine model dog showed no H-reflex (B). Both have normal M waves. The M wave amplitude showed no remarkable change after pyridoxine treatment, a finding which was confirmed in our statistical analysis (p < 0.05). There was a remarkable change in the H-reflex after treatment in the experimental group.

• 

  Fig. 2 (A) Normal dorsal funiculus in L4. (B) Dorsal funiculus in L4 showing disruption and irregularity of axons and myelin with vacuolation. (C) Normal dorsal root ganglia in L4. (D) Dorsal root ganglia in L4 showing chromatolysis with eccentric location of nucleus (arrow). H&E stain, ×200.

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  Fig. 3 (A) Normal sciatic nerve. (B) Sciatic nerve showing axonal swelling with vacuolation (arrows). (C) Normal tibial nerve. (D) Tibial nerve showing axonal swelling with vacuolation (arrows). H&E stain, ×400.

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