Exp Mol Med.
2004 Dec;36(6):588-592.
Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice
- Affiliations
-
- 1Laboratory of Human Genomics Korea Research Institute of Bioscience and Biotechnology (KRIBB) Daejeon 305-806, Korea. lee10@kribb.re.kr
- 2Department of Pathology and Institute of Medical Science Wonkwang University, School of Medicine Iksan 570-749, Korea.
- 3Department of Anatomy College of Medicine, Chungnam National University Daejeon 301-131, Korea.
- 4Department of Oral Biochemistry College of Dentistry, Chonnam National University Gwangju 500-757, Korea.
- 5Department of Microbiology College of Natural Sciences, Pusan National Unversity Pusan 609-735, Korea.
- 6College of Veterinary Medicine Chungnam National University Daejeon 305-764, Korea.
Abstract
- Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.
Keyword
MeSH Terms
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Amino Acid Sequence
Animals
Base Sequence
Gene Expression
Genetic Vectors/genetics
Hepatitis C/*pathology/virology
Hepatitis, Viral, Animal/*pathology/virology
Hepatocytes/pathology/virology
Liver/pathology/*virology
Mice
Mice, Transgenic
Molecular Sequence Data
Mutation/genetics
RNA, Messenger/chemistry/metabolism
Research Support, Non-U.S. Gov't
Transgenes
Viral Core Proteins/analysis/*genetics/metabolism
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