Exp Mol Med.
2008 Aug;40(4):361-369. 10.3858/emm.2008.40.4.361.
Regeneration of kidney tissue using in vitro cultured fetal kidney cells
- Affiliations
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- 1Department of Bioengineering, Hanyang University, Seoul, Korea. bskim@hanyang.ac.kr
- 2Immune Disease Institute, Harvard Medical School, Boston, USA.
- 3Department of Chemical Engineering, Hanyang University, Seoul, Korea.
- 4Department of Pathology, Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul, Korea.
- 5Department of Pathology, College of Medicine, Hanyang University, Seoul, Korea.
- KMID: 1094115
- DOI: http://doi.org/10.3858/emm.2008.40.4.361
Abstract
- Transplanting fetal kidney cells (FKCs) can regenerate kidney. This requires in vitro expansion in cell number to acquire enough cells for transplantation. However, FKCs may change their cellular characteristics during expansion and, thus, may not regenerate kidney tissue upon transplantation. We investigated how cell culture period affects cellular characteristics and in vivo regenerative potential of FKCs. As the passage number increased, cell growth rate and colony forming ability decreased while senescence and apoptosis increased. To examine in vivo regenerative potential, FKCs cultured through different numbers of passages were implanted into the parenchyma of kidneys of immunodeficient mice using fibrin gel for 4 wk. Histological analyses showed passage-dependent kidney tissue regeneration, and the regeneration was better when cells from lower number of passages were implanted. This result shows that in vitro culture of FKCs significantly affects the cell characteristics and in vivo tissue regenerative potential.
Keyword
MeSH Terms
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Animals
Apoptosis/physiology
Cell Aging/physiology
Cell Culture Techniques
Cell Proliferation
Cells, Cultured
Colony-Forming Units Assay
Female
Fetal Tissue Transplantation/methods/physiology
Fetus/cytology/*physiology
Kidney/embryology/*physiology
Mice
Mice, Inbred BALB C
Mice, Nude
Rats
Rats, Sprague-Dawley
Regeneration/*physiology
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