J Vet Sci.
2004 Dec;5(4):353-357.
Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus
- Affiliations
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- 1Department of Veterinary Virology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. parkx026@snu.ac.kr
- 2Department of Veterinary Infectious Disease, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
- 3Division of Biological Science and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Korea.
Abstract
- A few members of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea pigs, rabbits and sows. The animals (20 guinea pigs, 8 rabbits and 20 sows) were divided into 4 groups. Group A was injected intramuscularly (IM) with soluble pAPN at one hour before intramuscular infection of PEDV on the same site, group B for IM simultaneous injection of pAPN and PEDV, and group C for IM injection of PEDV only. Group D served as a control of pAPN treatment or PEDV infection. Antibody production against PEDV was compared among groups at regular intervals. The results suggested that pAPN could enhance the antibody production against PEDV in guinea pigs and rabbits which are free of pAPN, however, the effect of pAPN treatment in sows was not clearly elucidated.
MeSH Terms
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Animals
Antibodies, Viral/*blood
Antibody Formation
Antigens, CD13/*administration&dosage
Cercopithecus aethiops
Coronavirus/*immunology/physiology
Coronavirus Infections/immunology/*veterinary
Enzyme-Linked Immunosorbent Assay/veterinary
Female
Guinea Pigs
Immunoglobulin G/*blood
Immunoglobulin Isotypes
Injections, Intramuscular
Pregnancy
Rabbits
Solubility
Swine
Swine Diseases/*immunology
Vero Cells/virology
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